Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: UNIPROT:P41181 (
collecting duct
)
5,183
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
The
fragile histidine triad
(
FHIT
) gene located in human chromosome 3p14.2 is a candidate tumor suppressor gene that has a diadenosine triphosphate (Ap3A) hydrolase activity, but its role in carcinogenesis remains uncertain. To investigate the role of
FHIT
in normal cells, specific polyclonal antibodies to recombinant rat FHIT protein were generated. Immunoblot analysis revealed the 17-kd FHIT protein was most abundantly expressed in kidney and liver, whereas heart, skeletal muscle, and adrenal gland expressed trace amounts. In kidney, immunohistochemical staining was strongly observed in distal convoluted tubule and
collecting duct
during postnatal growth period. By a nested reverse transcription-PCR analysis of
FHIT
from 2 human kidney cancer cell lines, four abnormal-sized
FHIT
transcripts, with deletion and/or insertion, were detected. These were derived from the results of exon skipping, and/or insertion of
FHIT
intron 5 sequence, or selection of cryptic splice site within the
FHIT
cDNA sequence 179-180. Taken together, our data indicate that
FHIT
expression is frequently altered in human kidney cancer cell lines by alternative splicing, and suggest that the FHIT protein may play a pivotal role in regulating intracellular metabolism of the distal convoluted tubule and
collecting duct
in maturity.
...
PMID:Expression of the fragile histidine triad gene in normal rat tissues and human kidney cancer cell lines. 1508 May 5
