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Query: UNIPROT:P41181 (collecting duct)
 5,183 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Cell culture conditions were devised that selectively supported growth of 13 or 14 gestation day F344 rat ureteric bud, the renal collecting duct anlagen. These same conditions also inhibited the growth of metanephrogenic mesenchyme, precursor of structures proximal to the duct. Isolated buds were cultured in Ham's F12 medium supplemented with epidermal growth factor, selenium, insulin, hydrocortisone, prostaglandin E1, transferrin, and triiodothyronine; fetal bovine serum (1%) was required for continuous propagation. Cultured cells were epithelial in morphology and formed domes. By electron microscopy, many structural characteristics of highly differentiated cells were evident: numerous mitochondria, Golgi apparatus, extensive endoplasmic reticulum, an occasional cilium, intracytoplasmic filaments, polarized formation of microvilli, and gap junctions. Histochemistry revealed considerable functional differentiation as well. Cultured bud cells, adult collecting duct, and fetal duct anlagen were positive for acid phosphatase, membrane-localized ATPase, and nonspecific esterase. Bud cells and fetal duct anlagen expressed high levels of gamma-glutamyl transpeptidase activity while adult collecting duct exhibited slight activity. In addition, immunocytochemical observation of intermediate filament expression revealed the presence of epithelial cytokeratins but absence of mesenchymal vimentin in cultured bud cells and fetal and adult collecting ducts. These results indicate that the culture conditions described can maintain the partially differentiated fetal collecting duct anlagen in a state consistent with its embryonal derivation, and therefore may be useful in culture studies of renal differentiation.
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PMID:Selective growth in culture of fetal rat renal collecting duct anlagen. Morphologic and biochemical characterization. 286 2

Examination of light microscopical sections of the kidneys of guinea pigs with chronic exposure to mercury as the result of the breakdown of subcutaneous implants of powdered dental amalgam demonstrated the development of black, refractile deposits in the cytoplasm and nuclei of cells in both the straight and convoluted portions of the proximal tubule. The more numerous cytoplasmic deposits were of a particulate nature with dimensions of approximately 1 microgram. The nuclear deposits, which appeared later but which were relatively more common in longer-term animals, took the form of prominent inclusions, 1 to 3 micrograms in diameter. The ratio of nuclear to cytoplasmic deposits was higher in animals receiving high copper as compared with conventional amalgam. At electron microscopical level, the cytoplasmic deposits were seen to consist of collections of fine particles within lysosomes. Similar deposits were also found in far smaller numbers in lysosomes in collecting duct cells. The nuclear inclusions in proximal tubular cells were made up of closely packed electron dense granules. X-ray microanalysis showed both lysosomal and nuclear deposits to contain mercury and selenium. The association of mercury with selenium, which was present in the animals' diet at low levels, probably aided the microscopical visualisation of the deposits.
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PMID:The development of mercury- and selenium-containing deposits in the kidneys following implantation of dental amalgams in guinea pigs. 302 28

Selenium is an essential trace element for the maintenance of structures and functions of kidney. To evaluate the effects of low selenium on the kidneys of growing rats, newborn rats were fed with selenium deficient and normal diets respectively for 109 days. As a result, rats fed with low selenium diets resulted in a decline in the body weight and the concentration of selenium in the kidney, especially the male rats from the low selenium groups. Moreover, the ultrastructure of glomerulus and tubules were damaged in low selenium group: the glomeruli were observed with hyperplasia of mesangial cells, fusion of podocyte foot processes and thickening of basement membrane; and the tubules were observed with vacuolar degenerated epithelial cells, increased edema fluid or protein solution between cells, microvilli edema, increased cell gaps and decreased cell links. Furthermore, the pathological changes in selenium deficient group included the increase of fibers around renal hilum aorta and in the renal collecting duct, and shed of cells in the proximal convoluted tubules. In addition, up-regulated expressions of matrix metalloproteinases (MMP1/3) and down-regulated expressions of their inhibitors (TIMP1/3) at the mRNA and protein levels were also appeared to be relevant to low selenium. The results suggested that low selenium in diet may cause low selenium concentration in the kidney of growing rat and lead to damages of the ultrastructure and extracellular matrix (ECM) of kidney.
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PMID:Selenium deficiency induced damages and altered expressions of metalloproteinases and their inhibitors (MMP1/3, TIMP1/3) in the kidneys of growing rats. 2685 38