Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UNIPROT:P41181 (collecting duct)
 5,183 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

The biochemical and morphological extent of glycoprotein synthesis inhibition of cellular and extracellular proteins was studied on cultured renal collecting duct (CD) epithelium. We found that tunicamycin (4 micrograms/ml) inhibits the glycosylation of a 150,000 d glycoprotein (gpCDI). A 85,000 d glycoprotein (gpCDII) was not affected. The inhibition by tunicamycin demonstrates that gpCDI has characteristics of a N-glycan, whereas gpCDII seems to be an O-glycan. 6-diazo-5-oxo-norleucine (4 X 10(-5) M) which was used as glutamine analogue, did not show a comparable inhibitory effect as seen with tunicamycin. The lack of effect of norleucine demonstrates that glutamine is not the locus of glycosylation in both proteins. However, because of the tunicamycin inhibition it points to asparagine as the site of glycosylation in the gpCDI. Long term cultures of the tissue up to 15 days in the presence of tunicamycin and norleucine and of substances usually used as basement membrane inhibitors, such as hydroxy-D-proline (1 mM), L-azetidine-2-carboxylic acid (1 mM) and o- and p-nitrophenyl-xylopyranoside 1 mM), revealed that it is possible to eliminate completely the fibroblasts beneath the cultured epithelium and within the degenerating corematerial. Experiments with hydroxy-D-proline showed the most striking effect. Experiments with L-azetidine-2-carboxylic acid and nitrophenyl-xylopyranoside resulted in the elimination of fibroblasts and dedifferentiation of the collecting duct epithelium.
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PMID:Effects of glycoprotein and basement membrane synthesis inhibitors on the growth of cultured renal collecting duct epithelium. 648 Apr 14

Renal cortical explants consisting of capsula fibrosa with an adherent thin layer of collecting duct anlagen, S-shaped bodies, and nephrogenic blastema were isolated from newborn New Zealand rabbits and cultured in Dulbecco's Modified Eagle's medium for 24 hours. Within this period of time, the explants formed globular bodies surrounded by an epithelium of differentiated collecting duct cells. The outgrowth of the collecting duct cells and the formation of the epithelium occurred only when serum was added to the cultivation medium. Different types and different concentrations of serum were tested; fetal bovine serum and newborn and adult rabbit sera at concentrations of at least 5% induced the outgrowth and spreading of the cells. The surrounding epithelium did not develop in the absence of serum. The outgrowth of the collecting duct cells in serum-supplemented cultivation media was arrested by inhibitors of protein and glycoprotein synthesis and by cytoskeletonal-blocking agents such as cycloheximide (1 x 10(-6) M), actinomycin C1 (2 micrograms/ml), tunica-mycin (1 microgram/ml), 6-diazo-5-oxo-norleucine (2 x 10(-5) M), vinblastine (5 x 10(-6) M), colchicine (1 x 10(-3) M), and cytochalasin B (2 micrograms/ml). In contrast, inhibitors of DNA synthesis, e.g., cytosine arabinoside (2.5 x 10(-5) M), mitomycin (1 x 10(-6) M), and hydroxyurea (2.5 x 10(-3) M), had no influence on the outgrowth.
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PMID:Induction and inhibition of outgrowth and development of renal collecting duct epithelium. 684 85