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Query: UNIPROT:P41181 (collecting duct)
 5,183 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Inner medullary collecting duct cells were isolated from rat papillae and grown to confluence on cover slips. H+ secretion was estimated by intracellular pH (pHi) changes measured with the fluorescent probe 2,7-biscarboxyethyl-5(6)-carboxyfluorescein. In buffered NaCl, pHi was 7.14 +/- 0.04 (n = 78). After acidification about 40% of monolayers exhibited Na+-independent alkalinization. In 5 mM glucose, cell alkalinization occurred at a rate of 47 +/- 4 nM H+/min. However, cell alkalinization did not occur in the presence of 2-deoxy-D-glucose (5-15 mM), iodoacetate (5 mM), or KCN (5 mM). All monolayers tested exhibited amiloride-inhibitable Na+-dependent cell alkalinization that appeared to be a first-order kinetic process; Km [Na+] was approximately 52 mM and Vmax was approximately 250 nM [H+]/min. At a constant extracellular [Na+] (110 mM), Na+-dependent H+ efflux was a first-order function of pHi; Km for intracellular [H+] = 321 nM and Vmax = 182 nM H+/min. The data are consistent with the presence of a primary active H+ pump and a secondary active Na+ exchanger. The metabolic energy for the active H+ pump can be provided by glycolysis and oxidative phosphorylation.
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PMID:Mechanisms of H+ secretion by inner medullary collecting duct cells. 334 16

Fragments of rabbit cortical collecting tubule (CCT) were isolated by solid-phase immunoadsorption with a monoclonal antibody against an ectoantigen on principal cells. Primary cultures, initiated from the "immunodissected" cells, were then grown to confluency in porous bottom dishes. The purity of the freshly isolated and cultured cells was verified by flow cytometry and immunocytochemistry, using a panel of cell-specific monoclonal antibodies and lectins. As a result of continued vectorial transport, the cultured epithelial monolayers generated pronounced transepithelial solute gradients. The composition of the luminal (L) and basolateral (B) media after 48 h of incubation was as follows: [Na], L 119 +/- 1.1, B 145 +/- 3.0; [K], L 11.9 +/- 0.5, B 2.0 +/- 0.3; [PO4], L 2.8 +/- 0.2, B 7.3 +/- 0.3; [Cl], L 134.1 +/- 2.7 B 117.8 +/- 2.2, [mM]; [Ca], L 42.1 +/- 1.2, B 812 +/- 26, [microM]; pH, L 5.52 +/- 0.12, B 7.15 +/- 0.08. The transepithelial osmotic concentration difference was 14.3 +/- 3.5 mosmol/l. The amount of glucose at the luminal side remained unaltered, whereas glucose concentration at the basolateral side decreased from 21 to 7.7 +/- 1.0 mM. Lactic acid accumulated predominantly at the basolateral side (L 1.0 +/- 0.15, B 23.5 +/- 1.7 mM). These data indicate that primary cultures of immunodissected CCT cells retain many of their differentiated transport functions and thus should prove useful for studying the mechanisms of solute transport in the collecting duct system.
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PMID:Differentiated transport functions in primary cultures of rabbit collecting ducts. 342 33

To investigate the mechanisms responsible for urinary acidification in the terminal nephron, primary cultures of cells isolated from the renal papilla were grown as monolayers in a defined medium. Morphologically, cultured cells were epithelial in type, and similar to collecting duct principal cells. Cell pH measured fluorometrically in monolayers grown on glass slides showed recovery from acid loads in Na+-free media. Recovery was inhibited by cyanide, oligomycin A, and N-ethylmaleimide. Cyanide and oligomycin inhibited recovery less in the presence than in the absence of glucose. When cells were first acid loaded in a Na+-free medium and then exposed to external Na+, pH recovery also took place. This recovery exhibited first-order dependence on Na+ concentration and was inhibited by 5-(N-ethyl-N-isopropyl)amiloride. These studies demonstrate that in culture, collecting duct principal cells possess at least two mechanisms for acid extrusion: a proton ATP-ase and an Na+-H+ exchanger. The former may be responsible for some component of the urinary acidification observed in the papillary collecting duct in vivo; the role of the latter in acid-base transport remains uncertain.
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PMID:Regulation of pH in rat papillary tubule cells in primary culture. 368 May 19

Lactate production was measured directly in individual segments of the rat nephron. Tubules were dissected and then incubated in vitro with glucose as the only metabolic substrate. Each segment was incubated with and without antimycin A, an inhibitor of oxidative metabolism. Proximal tubules produced no lactate with or without antimycin A. The distal segments all produced lactate. The rate of lactate production without antimycin A ranged from 0.4 to 0.9 pmol X min-1 X mm-1 in all distal segments except one, the inner medullary collecting duct, which produced lactate at the significantly higher rate of 2.8 pmol X min-1 X mm-1. Antimycin A increased lactate production significantly in all of the distal segments. The increase was largest in medullary thick ascending limbs (1,400%) and cortical (798%) and outer medullary collecting ducts (357%). Increments were smaller in cortical thick ascending limbs (98%) and distal convoluted tubules (98%) and least in the inner medullary collecting ducts (28%). We conclude that lactate production occurs only in distal segments of the nephron and that under anoxic conditions significant amounts of ATP are produced by anaerobic glycolysis in these segments.
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PMID:Lactate production in isolated segments of the rat nephron. 398 59

Free-flow micropuncture studies were performed on twenty-seven young female Sprague-Dawley rats before and after 10% extracellular volume expansion to evaluate glucose reabsorption at the accessible sites of both surface and papillary nephrons. In the distal nephron segments no significant glucose reabsorption was observed for the distal tubule and papillary collecting duct but significant difference in fractional glucose delivery was demonstrated between the bend of the Henle's loop and early distal tubule and between the late distal tubule and the base of the collecting duct. Comparison of the fractional glucose delivery within the same nephron group for both superficial and juxtamedullary nephrons indicated that glucose reabsorption occurred at some sites beyond the bend of the Henle's loop. Volume expansion inhibited glucose reabsorption in the proximal convoluted tubule, enhanced it in the segment between the late proximal and early distal tubules, but had no effect on glucose transport at further distal sites. It is concluded that, in addition to the proximal tubule, the ascending loop of Henle or cortical collecting tubule may play a role in maintaining glucose-free urine under physiological conditions.
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PMID:Segmental analysis of renal glucose transport in young female rats. 639 45

Reabsorption of glucose, salt and water was measured in 7-8 day pregnant and virgin rats by micropuncturing distal nephrons during saline and glucose infusions. Unidirectional fluxes of glucose were measured in loops of Henle and collecting ducts. There were no significant differences in single nephron glomerular filtration rate (S.N.G.F.R.) between virgin and pregnant animals during saline infusion. During glucose infusion S.N.G.F.R. was higher in pregnant animals than in virgins. There is no evidence of a failure of reabsorption of glucose by the proximal tubule in pregnant animals. More glucose is reabsorbed from the loop of Henle in virgin animals than in pregnant animals during saline infusion but during glucose infusion the converse is true. During both saline and glucose infusion there is less reabsorption of glucose from the collecting duct in pregnant animals than in virgin animals. It is concluded that the increased excretion of glucose during pregnancy can be attributed to alteration of glucose handling by distal segments of the nephron.
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PMID:Glucose handling by distal portions of the nephron during pregnancy in the rat. 687 3

The early effects of lithium on the kidney were studied in rats receiving a moderate daily dose (serum-Li: 0.5 to 0.8 mM per liter) for 3, 7, and 21 days. Enzyme histochemical reactions for acid and alkaline phosphatase, glucose 6-phosphatase, succinate and alpha-glycerophosphate dehydrogenase, and NADH tetrazolium reductase revealed changes confined to distal convoluted tubules and collecting ducts. The distal convoluted were unchanged at 3 days of treatment. At 7 days, a decrease in succinate dehydrogenase and NADH tetrazolium reductase and an increase in alpha-glycerophosphate dehydrogenase were noted. These changes were more conspicuous at 21 days and accompanied by tubular dilation and changes in light microscopic cellular morphology. In the collecting ducts, a cell enlargement and an increase in mitochondrial oxidative enzyme activities began to appear at 3 days, becoming more pronounced at 7 and particularly at 21 days. At 7 and even more at 21 days, a cellular hyperplasia was evident in the collecting ducts, and autoradiography after 3H-thymidine incorporation showed a marked increase in DNA synthesis in the collecting duct cells. The changes observed in the collecting ducts were most pronounced near the limit between the outer and the inner zone of the medulla. In conclusion, the rats developed morphologic changes at 3 to 7 days of treatment. The changes include (1) signs of cellular damage in the distal convoluted tubules and (2) hyperplasia and signs of increased functional activity in the collecting ducts.
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PMID:Early changes in renal distal convoluted tubules and collecting ducts of lithium-treated rats: light microscopy, enzyme histochemistry, and 3H-thymidine autoradiography. 706 26

Antidiuretic hormone (ADH) regulates renal water excretion by altering the permeability of the collecting duct to water. ADH-responsive epithelial cells are the major cell type lining kidney tubules in the inner medulla and papilla. ADH modulates apical membrane water permeability by the insertion and removal of vesicles containing aquaporin collecting duct water channel protein (now termed AQP-2). To identify and characterize proteins responsible for trafficking of AQP-2-containing vesicles, we utilized antibody and cDNA probes to synaptobrevin b (also termed VAMP-2, for vesicle-associated membrane protein 2), a protein that mediates synaptic vesicle exocytosis in the brain and whose structural homologs are now considered to be components of a complex responsible for intracellular vesicle fusion in all cells. We now report that rat kidney inner medulla and papilla contain abundant synaptobrevin protein. Only light endosomes, one of two types of purified papillary AQP-2-containing endosomes, possess synaptobrevin. Light endosomes fuse in vitro by means of an ATP-dependent process that is significantly inhibited when endosomes are preincubated with either anti-synaptobrevin antibody or tetanus toxin. These data define a functional role for a synaptobrevin protein in the fusion of endosomes in vitro. The presence of abundant synaptobrevin proteins in endosomes containing AQP-2 water channels, as well as insulin-sensitive glucose transporters [Cain, C. C., Trimble, W. S. & Lienhard, G. E. (1992) J. Biol. Chem. 267, 11681-11684], and in cells of Malpighian tubules responsible for urine formation in insects [Chin, A. S., Burgess, R. W., Wong, B. R., Schwartz, T. L. & Scheller, R. H. (1993) Gene 131, 175-181] suggests a specialized role for synaptobrevin in vesicle-mediated membrane transport modulated by peptide hormones.
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PMID:Rat kidney papilla contains abundant synaptobrevin protein that participates in the fusion of antidiuretic hormone-regulated water channel-containing endosomes in vitro. 753 5

Renal oncocytomas, which have previously been shown to originate from the collecting duct system, were induced in male Sprague-Dawley rats by oral administration of N-nitrosomorpholine (NNM) for 7 weeks. The expression of glucose transporter isoforms GLUT1 and GLUT2, and of several enzymes involved in glucose metabolism [hexokinase (HK), pyruvate kinase (PK), lactate dehydrogenase (LDH), malate dehydrogenase (MDH)] were studied by cytochemical approaches in serial cryostat sections of the kidney 12, 23 and 34 weeks after withdrawal of NNM. Oncocytic tubules connected with collecting ducts were first observed 23 weeks, and oncocytomas 34 weeks after withdrawal. The cytochemical pattern of oncocytic tubules and oncocytomas was similar, but differed markedly from that of normal collecting ducts in nearly all variables studied; expression of GLUT1 and hexokinase I proteins were strongly increased; activities of HK, PK and MDH were elevated, while LDH activity was reduced. These results suggest that oncocytic transformation is associated with fundamental changes in energy metabolism which differ from those in cell lineages leading to other types of renal cell tumours, such as clear/acidophilic and basophilic cell tumours. The characteristic over-expression of GLUT1 may be used as a diagnostic criterion for the discrimination between oncocytes and acidophilic (granular) cells in clear/acidophilic renal cell tumours which show a reduced expression of this glucose transporter protein.
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PMID:Over-expression of glucose transporter isoform GLUT1 and hexokinase I in rat renal oncocytic tubules and oncocytomas. 792 15

Pregnancy in the rat is accompanied by enhanced reabsorption of salt and water throughout most, if not all, of the gestational period. Many mechanisms have been suggested but definitive answers are still awaited. The major area of controversy centres around the detection of changes at term. There is general agreement that, at least in mid-gestation, the increase in reabsorption can be attributed to increases in the proximal tubules, the loop of Henle and the collecting duct. The contribution of the proximal tubule to the increased reabsorption at term is still uncertain. Enhanced salt and water reabsorption is demonstrated in distal nephron segments irrespective of the stage of gestation. Micropuncture and microperfusion experiments have identified increased reabsorption of water, sodium and chloride in the loop of Henle, but it appears that there is net addition of glucose, urea and potassium to the tubular fluid in this segment which, at least for potassium and glucose, offsets to some extent increased reabsorption by the proximal tubule. Altered renal handling of other solutes (uric acid, calcium and magnesium) also occurs throughout pregnancy but the mechanisms responsible and nephron sites involved remain to be investigated. Attempts to attribute altered reabsorption to direct renal effects of changes in maternal hormones are inconclusive. Prolactin mimics some of the pregnancy-associated increases in reabsorption following chronic administration to male and non-pregnant female rats. These effects might be due to a direct renal action of the hormone or even to the volume expansion following its dipsogenic action.
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PMID:Renal tubular function in the gravid rat. 792 8


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