Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: UNIPROT:P41181 (
collecting duct
)
5,183
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
A Ca
2+
-activated nonselective cation channel (NSC
Ca
) is found in principal cells of the mouse cortical
collecting duct
(
CCD
). However, the molecular identity of this channel remains unclear. We used mpkCCD
c14
cells, a mouse
CCD
principal cell line, to determine whether NSC
Ca
represents the transient receptor potential (TRP) channel, the melastatin subfamily 4 (
TRPM4
). A Ca
2+
-sensitive single-channel current was observed in inside-out patches excised from the apical membrane of mpkCCD
c14
cells. Like
TRPM4
channels found in other cell types, this channel has an equal permeability for Na
+
and K
+
and has a linear current-voltage relationship with a slope conductance of ~23 pS. The channel was inhibited by a specific
TRPM4
inhibitor, 9-phenanthrol. Moreover, the frequency of observing this channel was dramatically decreased in
TRPM4
knockdown mpkCCD
c14
cells. Unlike those previously reported in other cell types, the
TRPM4
in mpkCCD
c14
cells was unable to be activated by hydrogen peroxide (H
2
O
2
). Conversely, after treatment with H
2
O
2
,
TRPM4
density in the apical membrane of mpkCCD
c14
cells was significantly decreased. The channel in intact cell-attached patches was activated by ionomycin (a Ca
2+
ionophore), but not by ATP (a purinergic P
2
receptor agonist). These data suggest that the NSC
Ca
current previously described in
CCD
principal cells is actually carried through
TRPM4
channels. However, the physiological role of this channel in the
CCD
remains to be further determined.
...
PMID:Hydrogen peroxide suppresses TRPM4 trafficking to the apical membrane in mouse cortical collecting duct principal cells. 2795 81
