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Query: UNIPROT:P41181 (
collecting duct
)
5,183
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Transient receptor potential cation channel subfamily V member 4
(
TRPV4
)-mediated Ca
2+
signaling induces early activation of small/intermediate Ca
2+
-activated K
+
channels, SK3 (KCNN3) and IK1 (KCNN4), which leads to membrane hyperpolarization and enhanced Ca
2+
influx, which is critical for subsequent activation of the large conductance Ca
2+
-activated K
+
channel BK (KCNMA1) and K
+
secretion in kidney cortical
collecting duct
(
CCD
) cells. The focus of the present study was to determine if such coordinated hierarchical/sequential activation of these channels in
CCD
was orchestrated within caveolae, a known microcompartment underlying selective Ca
2+
-signaling events in other cells. In K
+
-secreting mouse principal cell (PC) line, mCCDcl1 cells, knockdown of caveolae caveolin-1 (CAV-1) depressed
TRPV4
-mediated Ca
2+
signaling and activation of SK3, intermediate conductance channel (IK1), and BK. Immunofluorescence colocalization analysis and coimmunoprecipitation assays demonstrated direct coupling of
TRPV4
with each of the KCa channels in both mCCDcl1 and whole mouse kidney homogenates. Likewise, extending this analysis to CAV-1 demonstrates colocalization and direct coupling of CAV-1 with
TRPV4
, SK3, IK1, and BK, providing strong support for coupling of the channels in caveolae microdomains. Furthermore, differential expression of CAV-1 along the
CCD
was apparent where CAV-1 was strongly expressed within and along the cell borders of kidney PCs and intercalated cells (ICs), although significantly less in ICs. It is concluded that caveolae provide a key microdomain in PCs and ICs for coupling of
TRPV4
with SK3, IK1, and BK that directly contributes to
TRPV4
-mediated Ca
2+
signaling in these domains leading to rapid and sequential coupling of
TRPV4
-SK3/IK1-BK that may play a central role in mediating Ca
2+
-dependent regulation of BK and K
+
secretion.
...
PMID:Caveolae facilitate TRPV4-mediated Ca
2+
signaling and the hierarchical activation of Ca
2+
-activated K
+
channels in K
+
-secreting renal collecting duct cells. 3020 67
