Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: UNIPROT:P41002 (
CCNF
)
32
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Parthenotes have been proposed as a source of embryonic stem cells but they lack the centriole which is inherited through the sperm in all mammalian species, except for rodents. We investigated the centrosome of parthenotes and parthenogenetic embryonic stem cells using parthenogenetic and biparental pig pre-implantation embryos, human and pig parthenogenetic and biparental embryonic stem cells, sheep fibroblasts derived from post implantation parthenogenetic and biparental embryos developed in vivo. We also determined the level of aneuploidy in parthenogenetic cells. Oocytes of all species were activated using ionomycin and 6-dimethylaminopurine (6-DMAP). Over 60% of parthenogenetic blastomeres were affected by an excessive number of centrioles. Centrosome amplification, was observed by microscopical and ultrastructural analysis also in parthenogenetic cell lines of all three species. Over expression of PLK2 and down regulation of
CCNF
, respectively involved in the stimulation and inhibition of centrosome duplication, were present in all species. We also detected down regulation of spindle assembly checkpoint components such as BUB1, CENPE and MAD2. Centrosome amplification was accompanied by multipolar mitotic spindles and all cell lines were affected by a high rate of aneuploidy. These observations indicate a link between centrosome amplification and the high incidence of aneuploidy and suggest that parthenogenetic stem cells may be a useful model to investigate how aneuploidy can be compatible with cell proliferation and differentiation.
Stem Cell Rev Rep 2012
Dec
PMID:Centrosome amplification and chromosomal instability in human and animal parthenogenetic cell lines. 2266 Nov 17
We prepared carbon nanofiber (
CCNF
) using cellulose as the carbon source in this study and utilized for the first time as the support to enhance the catalytic performance of the cobalt ferrite (CoFe
2
O
4
) for peroxymonosulfate (PMS) activation. The catalytic capability of the CoFe
2
O
4
/
CCNF
nanocomposites activated PMS was investigated through degrading dimethyl phthalate (DMP), a classical organic pesticide pollutant, in water media. The influence factors like
CCNF
content, nanocomposite and PMS dosage, DMP content, and pH value on the degradation speed were systematically investigated and analyzed. Since CoFe
2
O
4
is a spinel structured molecule which is magnetically separable, the reusability of the prepared CoFe
2
O
4
/
CCNF
nanocomposites under multiple cycles was also tested. Besides, the degradation intermediates during the catalytic process were also analyzed and identified by liquid chromatography-mass spectrometry (LC-MS) with a possible degradation mechanism. The results indicated that the prepared nanocomposite had promising catalytic capability in degrading DMP, in which the SO
4
-
radicals played the main role as the active oxidation agent. Furthermore, the CoFe
2
O
4
/
CCNF
nanocomposites exhibited very good stability and reusability. The present study provides a clean biochar supported catalyst which could readily enhance the PMS activation efficiency for recycled decontamination of refractory organic pollutants in water media.
Sci Total Environ 2019
Dec
01
PMID:Cellulose derived carbon nanofiber: A promising biochar support to enhance the catalytic performance of CoFe
2
O
4
in activating peroxymonosulfate for recycled dimethyl phthalate degradation. 3138 55
