Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: UNIPROT:P39060 (
endostatin
)
2,284
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Endostatin is a 20 kDa carboxyl-terminal fragment of
collagen XVIII
that, when added exogenously, inhibits endothelial proliferation and migration in vitro and angiogenesis and tumor growth in vivo. Previous results showed
endostatin
/
collagen XVIII
labeling in few endothelial cells in human glioblastoma multiforme. We have now observed constitutive release of
endostatin
from one of four endothelial cell lines. Induction of
endostatin
release was observed after H2O2, an in vitro model of cell stress, CoCl2, a model of hypoxia, and by IFN-gamma challenge. Endostatin expression and release was reduced by the nitric oxide synthase inhibitors aminoguanidine and L-NAME and induced by the NO synthase-independent NO donors sodium nitroprusside (SNP) and spermine-NONO-ate. SNP-mediated
endostatin
induction was abrogated by the soluble guanylate cyclase inhibitor 1H-(1.2.4) oxadiazolo (4,3-A) quinoxalin-1-one. Adenoviral
endostatin
transduction resulted in the release of
endostatin
from endothelial cells and in down-regulation of iNOS (NOS2) and eNOS (
NOS3
), and surprisingly in a 10% induction of PCNA. These results describe the modulation of
endostatin
release by the NO signaling cascade and provide important new pharmacological information for the systemic induction of endogenous
endostatin
release by common NO donor pharmacotherapy.
...
PMID:Endothelial endostatin release is induced by general cell stress and modulated by the nitric oxide/cGMP pathway. 1283 91
