Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: UNIPROT:P39060 (
endostatin
)
2,284
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Arachidonic acid
(
ARA
) is a valuable polyunsaturated fatty acid produced by Mortierella alpina. Although some strategies such as nitrogen supplementation have shown the potential to affect the aging of M. alpina in ways which enable it to produce more
ARA
, the underlying mechanism remains elusive. Herein, we conducted a systematical analysis of the lipid droplet proteome, as well as the whole-cell proteome and metabolome, in order to elucidate how and why two different nitrogen sources (
KNO
3
and urea) affect the aging of M. alpina and the corresponding
ARA
concentration. We found that
KNO
3
promoted the
ARA
concentration, while urea accelerated lipid consumption and stimulated the decomposition of mycelia. Although both
KNO
3
and urea activated carbohydrate metabolic pathways,
KNO
3
exerted a stronger promoting effect on the pentose phosphate pathway and induced the lipid droplets to participate in the citrate-pyruvate cycle. The activities of malic enzyme and isocitrate dehydrogenase were also promoted more by
KNO
3
. These pathways provided additional substrates and reducing power for
ARA
synthesis and ROS elimination. Accordingly, since urea showed a weaker promotion of the related pathways, it caused a depression of the antioxidant system and a consequent increase of ROS. These findings facilitate the design of nitrogen supplementation strategies to achieve higher
ARA
concentrations, and provide guidance for deciphering the mechanisms of similar aging phenomena in other oleaginous microorganisms.
...
PMID:How nitrogen sources influence Mortierella alpina aging: From the lipid droplet proteome to the whole-cell proteome and metabolome. 2956 93
