Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: UNIPROT:P39060 (
endostatin
)
2,284
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
We previously reported that
endostatin
inhibits endothelial and tumor cellular invasion by blocking activation and catalytic activity of matrix metalloproteinase (MMP)-2. Here we have examined the domain of proMMP-2 responsible for the binding of
endostatin
using surface plasmon resonance. ProMMP-2 and proMMP-2deltaHP lacking the hinge and
hemopexin
-like (HP) domains bound little to the immobilized
endostatin
. The active MMP-2 and MMP-2deltaHP, but not the HP domain of MMP-2, bound to
endostatin
at similar levels. In addition, preincubation of MMP-2 and MMP-2deltaHP with the MMP inhibitor actinonin, which binds to the active site of MMP-2, abolished their bindings to
endostatin
. These results indicate that
endostatin
binds to neither the latent proMMP-2 nor the HP domain but to the catalytic domain of MMP-2.
...
PMID:Endostatin binds to the catalytic domain of matrix metalloproteinase-2. 1202 34
