Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UNIPROT:P30536 (PBS)
 9,886 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

The natural mechanisms of the body cannot control massive hemorrhaging, resulting in a requirement for hemostatic intervention. In this study, Graphene oxide and Chitosan aerogels reinforced with grape seed (SD) and skin (SK) extracts were developed for use as hemostatic agents by evaluating the influence of pH on their synthesis, and the amount of grape extract added on the physical and chemical properties of the aerogels. The material was evaluated by FTIR, XRD, Raman spectroscopy, DLS, uniaxial compression tests and SEM. The capacity of the aerogels to absorb water, PBS and blood, as well as their coagulation capacity, were determined. In addition, the release profile for grape extracts in PBS and the material's cytotoxicity were determined. The aerogels that were synthesized under basic conditions and loaded with grape extracts were more rigid and negatively charged, and they presented smaller pores than the un-loaded acidic aerogels. For all aerogels, the hemoglobin absorption was greater than 90 % in the first 30 s. A higher density of adsorbed blood cells was observed on aerogels loaded with a higher amount of grape extract. The maximum release of extract from the aerogels occurred for those loaded with SK extracts at a basic pH; the aerogels that were prepared under acidic conditions dissolved in the media. Aerogels loaded with SK extracts under alkaline conditions were not cytotoxic toward human dermal fibroblasts and exhibited cell viabilities above 90 %. These findings suggest that these aerogels have the potential for use as hemostatic agents in wound management.
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PMID:Synthesis and characterization of graphene oxide chitosan aerogels reinforced with flavan-3-ols as hemostatic agents. 3303 9

Herein, a new type of surface cellular protein imprinted polymers (MIPs) with inner macropores was fabricated via surface imprinting technology based on surface-modified Metal Organic Framework (MMOF-808) stabilized Pickering emulsion polymerization, and the MIPs were further used for selective adsorption and separation of bovine hemoglobin (BHb). For the first time, silane coupling agent modified MOF-808 particles were applied to stabilize a O/W emulsion, followed by the self-assembly of dopamine in the presence of BHb in the outer phase (PBS solution). SEM images proved that the MIPs possessed cellular structures, and the lotus seedpod-like structure could encourage more imprinted sites distributed on the surface of the polymeric materials, bringing about the imprinted sites easy accessibility. The static adsorption behaviors followed the Scatchard model with an equilibrium adsorption capacity of 406 mg g-1 and pseudo-first-order kinetics with the equilibrium adsorption time of 50 min. Moreover, the cellular polymers exhibited a prominent imprinting effect possessing the imprinting factor of 4.56. Importantly, the polymeric materials could be repeatedly used for rebinding bovine hemoglobin without significant loss in adsorption capacity. Therefore, the proposed approach we described in this work will provide a good reference in the separation and enrichment of biomacromolecules in aqueous solution.
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PMID:Lotus seedpod-like molecularly imprinted polymers fabricated by MOF-808 stabilized Pickering emulsion and their specific recognition of hemoglobin. 3316 Feb 60


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