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Query: UNIPROT:P30536 (PBS)
 9,886 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

A simple method for the preparation of a potent group-specific antigen on HeLa-229 cells infected with MRC-1 (LB) (TRIC/GB/MRC-1 Gf) strain of Chlamydia trachomatis is outlined. HeLa-229 cells are infected (MRC-1 strain, 102 inclusion-forming units per cell) with centrifugation at 4,000 g for 1 h in flat-bottomed vials. The cells are removed by brief trypsinization with 0.25% trypsin and put into 75 cm2 culture flasks (12 x 106 cells by flask) in BHK-21 medium supplemented with foetal bovine serum. The flasks are incubated for 5 days at 37 degrees C. The destroyed cell monolayer and the supernatant are centrifuged at 100,000 g for 1 h. The pellet is collected and resuspended in PBS and subjected to ultrasonic vibration for 30 min. This antigen may be used for detection of complement-fixing antibodies in lymphogranuloma venereum and ornithosis.
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PMID:[A method for the preparation of a Chlamydiae group-specific antigen on hela-229 cells infected with a strain of "Chlamydia trachomatis" for use in the complement fixation test (author's transl)]. 53 72

The blood was collected on filter paper, dried, put into an envelope and posted to the laboratory. One drop of blood extracted with 1 ml PBS was satisfactory for detecting natural cases of encephalitozoonosis by the india-ink immunoreaction.
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PMID:Diagnosis of encephalitozoonosis: a simple method for collection of rabbit blood. 55 90

Monolayer cultures of normal and diethylnitrosamine-transformed rat liver cells were labeled in situ with Con A-HRP or ferritin-conjugated Con A. Ligand-induced redistribution with simultaneous internalization of labeled membrane areas occurred in normal as well as in transformed cells when they were reincubated with PBS at 37 degrees C for different periods of time (from 5 min up to 3 hrs). Compared to normal cells, these afore mentioned processes were accelerated in transformed cells. Internalization in normal and transformed cells resulted in a recycling of labeled plasma membrane areas in the Golgi region with the label being finally accumulated in elements which correspond mostly, but not exclusively, to GERL. Then formation of phagolysosomes and multivesiculated bodies occurred whose labeled content was exocytized after fusion with the plasma membrane. This suggested that the internalized plasma membrane areas were at least partly degraded. The relabeling of some parts of the plasma membrane by extruded lysosomal content indicates that at least some Con A molecules are still biological active. Membrane internalization by endocytosis after binding of Con A obviously causes an increased of membrane biogenesis and exocytosis, thus compensating for membrane removal. This is suggested by the vacuolization and enlargement of unlabeled (not in recycling involved) Golgi apparatus. It may indicate a differential functional role of the Golgi apparatus in membrane turnover in the same cell. The fusion of phagolysosomes with the plasma membrane and the insertion of phagolysosomal membrane into the plasma membrane might be another compensatory mechanism.
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PMID:Cellular responses to surface binding and internalization of concanavalin A. An electron microscopic investigation on the problem of membrane cycling. 56 67

An hCG-like material has been extracted from human sperm. These experiments were designed to characterize this material. Sperms of 10 volunteers were separated from seminal fluid, washed in PBS three times, and resuspended in 0.5 ml of the same buffer. Samples were pooled; cells were disrupted by sonication and extracted in alkaline buffer by constant agitation at 4 degrees C. The extract was ultracentrifuged at 4 degrees C. Supernate was lyophilized and reconstituted in 2 cc of distilled water. This material presented a dose-response curve parallel to those of IS2-hCG and CR119 in beta hCG RIA. When chromatographed in a Sephadex G-150 column the extract eluted within the hCG range and immunoreacted in the specific beta hCG RIA. When absorbed onto a concanavalin A--Sepharose column, all recovered immunoreactive material eluted after exposure to alpha-D-methylglucoside, indicating that it is a glycoprotein. The extract stimulated progesterone and testosterone secretion in porcine granulosa cells and decapsulated rat testis, respectively, indicating its biologic potency.
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PMID:Presence of a human chorionic gonadotropin--like substance in human sperm. 57 20

72 strains of 24 Bacillus species were induced with mitomycin C. The lysates were examined for the presence of defective phages resembling PBS X in morphology. All strains tested of B. amyloliquefaciens. B, licheniformis, B. pumilus and B. subtilis contained such phages. Five morphological types of defective, PBS X-like phage could be distinguished, differing in their tail lengths and in the number of cross-striations on the tail. The quaternary structure of the tail, the molecular weight of the main tail protein and the antigenic properties of the phages were identical. The killing ranges of the defective phages have been determined and their possible use in taxonomy discussed.
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PMID:The occurrence and taxonomic value of PBS X-like defective phages in the genus Bacillus. 58 43

Murine peritoneal macrophages exhibit numerous slender microvilli and several invaginations of the cell membrane. By treatment with isotonic 2.10(-2) M procain-PBS the surface of macrophages is smoothed significantly. Isolated macrophages agglutinate opsonized isologous erythrocytes. Procainizing of macrophages inhibits this agglutination. The finding provides evidence for typing of macrophage receptors whether or not sensitive to procain treatment.
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PMID:Procainized murine macrophages fail in the agglutination of opsonized isologous erythrocytes. 59 Apr 23

RBC membrane polypeptide aggregates have been quantitated by PAGE SDS and by gel filtration. Aggregates were absent in fresh RBC's from normal controls, but aggregates with MW 4.4 X 10(5) and greater than 50 X 10(6) increased progressively as GSH levels fell in RBC's incubated in PBS without added glucose or calcium. Aggregates of both MW ranges were also present in fresh RBC's from a patient with compensated congenital nonspherocytic hemolysis associated with a mutant RBC G-6-PD, Long Prairie. Since the aggregates were dissociable by treatment with mercaptoethanol or dithiothreitol, they are probably cross-linked by intermolecular disulfide bonds. Membranes containing these aggregates may represent an early and sensitive indicator of oxidative damage to red cells.
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PMID:Membrane polypeptide aggregates in glucose 6-phosphate dehydrogenase-deficient and in vitro aged red blood cells. 62 30

Day 7 cow embryos were frozen in 1.5 M-DMSO in PBS at 0.3 degrees C/min to -36 degrees C and at 0.1 degrees C/min between -36 and -60 degrees C before being plunged directly into liquid nitrogen. They were subsequently thawed (rapidly to -50 degrees C, at 4 degrees C/min from -50 to -10 degrees C, and rapidly again) to room temperature. Embryonic viability was tested by four different transfer techniques. Maximum pregnancy rate (8/12) was obtained with surgical transfer immediately after thawing and dilution of DMSO.
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PMID:The viability of deep-frozen cow embryos. 63 24

A method of separating lymphoid cells from solid mouse mammary tumors was developed and evaluated. In this method the tumors are digested with 0.01% collagenase, 0.01% DNAase, and 0.025% trypsin in Dulbecco's PBS into suspensions of cells with a viability of 90%. The suspensions are fractionated on a continuous gradient of Ficoll in tissue culture medium. In model experiments this gradient was found to separate, cleanly, admixed cells of an established mammary tumor cell line and dissociated thymus glands. Recovery rates were 50% for the tumor cells and 80% for the thymocytes. The preparation of the cell suspensions and the gradient separation procedure are not harmful to the cells as indicated by trypan blue exclusion and the ability to grow in cell culture.
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PMID:In situ lymphoid cells of mouse mammary tumors. I. Development and evaluation of a method for the separation of lymphoid cells from mouse mammary tumors. 65 81

Formation of rosettes with sheep RBC by blood lymphocytes from young pigs was increased from 24.4% +/- 2.2 (mean +/- S.E.) in PBS to 54.1% +/- 1.9 in the presence of dextran. This increase was achieved without inducing appreciable rosette formation with other RBC which do not form rosettes in PBS. Lymphocytes which rosette only in dextran are predominant in pigs between 20 and 160 days old, when the peripheral lymphocyte pool is increasing very rapidly. The use of dextran revealed major populations of blood lymphocytes rosetting with SRBC in adult sheep (30.7% +/- 2.0), adult cattle (37.3% +/- 4.2) and adult goats (13.3% +/- 1.2). Proportions of rosette-forming lymphocytes tended to increase with age. In calf lymphoid tissues the distribution of rosette-forming lymphocytes suggested that these were T cells. In the improvement of rosette formation with SRBC, dextran was more effective than foetal calf serum, papain treatment of the SRBC or combinations of these treatments.
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PMID:Sheep erythrocyte rosettes in pigs, sheep, cattle and goats demonstrated in the presence of dextran. 67 Jul 10


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