Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: UNIPROT:P30536 (PBS)
 9,886 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Heparin (Hep), widely used in clinics as an anticoagulant drug, has high degrees of heterogeneity and shares a similar disaccharide repeating unit with its GAG analogues. The development of reliable and convenient methods to discriminate Hep from its GAG analogues and detect trace GAG contaminants in Hep is meaningful for safe usage of Hep in clinics. Herein, five porphyrin-GO nanocomposites denoted as PP1-GO, PP2a-GO, PP2b-GO, PP3-GO, and PP4-GO were synthesized by assembling corresponding positively charged porphyrins onto the surface of GO. Controlled by a different number and position of the 4-N-methyl-pyridyl groups substituted at the porphyrins, these nanocomposites were determined to be cross-reactive toward Hep and other three commonly used GAGs including Chs, HA, and DS. A NIR sensor array PP-GO was thus constructed using these nanocomposites for GAGs discrimination and Hep quality control through pattern-based recognition. HCA and LDA calculated results indicated that PP-GO was powerful for discrimination of Hep and its GAG analogues in both PBS and even 10% serum media. Moreover, the PP-GO sensor array was successfully applied for the reliable discrimination of trace GAG contaminants in Hep with 100% accuracy.
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PMID:Porphyrin-GO Nanocomposites Based NIR Fluorescent Sensor Array for Heparin Sensing and Quality Control. 3226 69

Accurate discrimination of common glycosaminoglycans (GAGs) before they are used in clinics is of great importance. Herein, a ratiometric sensor array Py-PP for discrimination of GAGs was constructed using three pyrene-porphyrin supramolecular complexes termed Py-PP1, Py-PP2 and Py-PP4. These complexes were readily synthesized by mixing pyrene-1-butyric acid (Py) and porphyrins PP1, PP2 and PP4 respectively. In the presence GAGs, the effective FRET from Py to porphyrin in the complex was influenced as a result of the competitive binding interactions between porphyrin and GAG. Controlled by the structural differences in the three porphyrins, complexes Py-PP1, Py-PP2 and Py-PP4 were determined to be cross-responsive towards tested GAGs including Hep, HA, Chs and DS. Distinctive fluorescence patterns were successfully generated for each GAG by the sensor array. The Py-PP sensor array was found to be powerful for discrimination of GAGs in both PBS and 5% serum media. Moreover, Py-PP was also successfully applied for reliable differentiation of Hep from other biological interferences and detection of trace GAG contaminants (0.1%, wt%) in Hep with 100% accuracy.
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PMID:Pyrene-porphyrin based ratiometric fluorescent sensor array for discrimination of glycosaminoglycans. 3324 55