Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: UNIPROT:P30536 (
PBS
)
9,886
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Genetically engineered neural stem cell (NSC) lines are promising vectors for the treatment of regenerative diseases, especially Parkinson's disease (PD).
Neurturin
(
NTN
), a member of the glial cell line-derived neurotrophic factor-family, has been demonstrated to act specifically on mesencephalic dopaminergic neurons, suggesting its therapeutic potential for PD. Here, we have generated a
NTN
-secreting c17.2 NSC line and investigated the protective effect of
NTN
-c17.2 on PD rat models. These
NTN
-releasing NSCs engrafted and integrated in the host striatum with good success, gave rise to neurons, astrocytes and oligodendrocytes, and maintained stable, high-level
NTN
expression. In addition, inverse transfer of
NTN
protein into the substantia nigra (SN) was able to protect dopaminergic neurons from 6-OHDA toxicity. Observation of rotational behavior showed that the
NTN
group performed significantly better than the Mock group, and the protective effect of
NTN
lasted for at least 4 months. HPLC tests indicated that the contents of neurotransmitters (e.g. dopamine) in the corpus striatum area of the
NTN
-c17.2 group and the Mock-c17.2 group were significantly higher than in the
PBS
group, but there was no significant difference between expression in the
NTN
-c17.2 and Mock-c17.2 groups. Taken together, our results suggest that transplantation of
NTN
-secreting NSCs exerted protective on PD rat models.
...
PMID:Dopaminergic neuroprotection by neurturin-expressing c17.2 neural stem cells in a rat model of Parkinson's disease. 1696 9
