Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: UNIPROT:P30536 (
PBS
)
9,886
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
The study describes the development of a vaccine using microcrystalline cellulose (
Avicel PH
-101) as a delivery carrier of recombinant protein-based antigen against erysipelas. Recombinant SpaA, surface protective protein, from a gram-positive pathogen
Erysipelothrix rhusiopathiae
was fused to a cellulose-binding domain (CBD) from
Trichoderma harzianum
endoglucanase II through a S3N10 peptide. The fusion protein (CBD-SpaA) was expressed in
Escherichia coli
and was subsequently bound to
Avicel PH
-101. The antigenicity of CBD-SpaA bound to the Avicel was evaluated by enzyme-linked immunosorbent (ELISA) and confocal laser scanning microscope (CLSM) assays. For the examination of its immunogenicity, groups of mice were immunized with different constructs (soluble CBD-SpaA, Avicel coated with CBD-SpaA, whole bacterin of
E. rhusiopathiae
(positive control), and
PBS
(negative control)). In two weeks after immunization, mice were challenged with 1x10
7
CFU of
E. rhusiopathiae
and Avicel coated with CBD-SpaA induced protective immunity in mice. In conclusion, this study demonstrates the feasibility of microcrystalline cellulose as the delivery system of recombinant protein subunit vaccine against
E. rhusiopathiae
infection in mice.
...
PMID:Microcrystalline Cellulose for Delivery of Recombinant Protein-Based Antigen against Erysipelas in Mice. 2999 62
