Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UNIPROT:P30536 (PBS)
 9,886 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

A human polymorphonuclear leukocyte (PMN) activating factor (AFH-S) was isolated from a tropical plant seed, Aleurites Fordii Hemsl. (AFH) in PBS. It is found that the AFH-S stimulate human PMNs and induced superoxide generation and chemotaxis. Superoxide generation was affected by the extracellular calcium ion or pretreatment with H-7 (PK-C inhibitor), but not by mepacrine (PLA2 inhibitor) or pertussis toxin (islet-activating protein: IAP). Furthermore, a lag time exists dose-dependently. In addition the cytosolic calcium was not increased by the stimulation with AFH-S. Thus, the receptor for AFH-S was suggested to be independent from Ni-like protein, PI-response, or PLA2-activation, and stimulate PMNs through activation of PK-C.
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PMID:Human polymorphonuclear leukocyte activating factor isolated from Aleurites Fordii Hemsl. (Euphorbiaceae) seed. 285 81

We have used an irradiation and fusion procedure to generate somatic cell hybrids that maintain fragments of human chromosome 12. To identify hybrids retaining human material we performed repeat element-mediated PCR (intra-ALU PCR) on DNA extracted from individual cell clones. Twenty-five of 38 hybrids tested were shown to contain human material at low passage. These were further characterized by inter-ALU and inter-LI PCR. FISH analysis, using human Cot1 DNA as a probe, revealed the presence of 1-2 fragments in 11 out of 12 hybrids. The majority of the hybrids, 8 out of 12, were found to be homogeneous. The hybrid panel was characterized by 18 markers with known locations covering the entire chromosome 12. Thirteen of the 25 hybrids were positive for at least one marker. The results indicated the following order of loci on 12q: Cen-WNTI-A2MR-RAP1B-D12S8-PTBR-ATP2B1++ +-D12S7-D12S52-IGF1-PAH-PLA2-IFNG-ALDH2-te r. Three hybrids studied in more detail were found to carry overlapping fragments from chromosome segment 12q12-->q14.
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PMID:Isolation and characterization of radiation hybrids for human chromosome 12. 769 22

The pathogenesis of interstitial cystitis/painful bladder syndrome (IC/PBS) is multifactorial, but likely involves urothelial cell dysfunction and mast cell accumulation in the bladder wall. Activated mast cells in the bladder wall release several inflammatory mediators, including histamine and tryptase. We determined whether mitogen-activated protein (MAP) kinases are activated in response to tryptase stimulation of urothelial cells derived from human normal and IC/PBS bladders. Tryptase stimulation of normal urothelial cells resulted in a 2.5-fold increase in extracellular signal regulated kinase 1/2 (ERK 1/2). A 5.5-fold increase in ERK 1/2 activity was observed in urothelial cells isolated from IC/PBS bladders. No significant change in p38 MAP kinase was observed in tryptase-stimulated normal urothelial cells but a 2.5-fold increase was observed in cells isolated from IC/PBS bladders. Inhibition of ERK 1/2 with PD98059 or inhibition of p38 MAP kinase with SB203580 did not block tryptase-stimulated iPLA2 activation. Incubation with the membrane phospholipid-derived PLA2 hydrolysis product lysoplasmenylcholine increased ERK 1/2 activity, suggesting the iPLA2 activation is upstream of ERK 1/2. Real time measurements of impedance to evaluate wound healing of cell cultures indicated increased healing rates in normal and IC/PBS urothelial cells in the presence of tryptase, with inhibition of ERK 1/2 significantly decreasing the wound healing rate of IC/PBS urothelium. We conclude that activation of ERK 1/2 in response to tryptase stimulation may facilitate wound healing or cell motility in areas of inflammation in the bladder associated with IC/PBS.
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PMID:Tryptase activation of immortalized human urothelial cell mitogen-activated protein kinase. 2392 67