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Swine tongues were used as a model for evaluation of culture methods for Y. enterocolitica O3. The following three methods were used: Method A consisting of primary culture on DC, McConkey, CIN and SSD agar with additional long-term cold enrichment in PBS and/or selenite broth. Method B--2 min, treatment of samples with 0.5 per cent KOH solution followed by the same cultivation as above and Method C--"cold shock" i.e. incubation of swabs without any medium at -20 degrees C overnight and then inoculated in selenite broth and/or PBS. Methods B and C were approximately twice as effective as Method A. The percentage of detectability ranged from 70 to 90 per cent. CIN agar yielded 20 of 23 positive results when compared with other solid media. A total of 247 smears from pig tongues yielded 60 positive Y. enterocolitica O3 isolations i.e. 27 per cent on average.
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PMID:Comparative study of culture methods to detect Yersinia enterocolitica serogroup O3 on swine tongues. 233 Dec 97

The purpose of the study was to determine the occurrence of Yersinia enterocolitica in tissues of aborted fetuses, placentas, vaginal and rectal swabs of aborting sows from pig farms where reproductive disturbances were found and to determine and analyze the biotype and serotype affinity of the strains isolated. Altogether 97 fetuses aborted in various stages of pregnancy, 25 placentas and swabs from 231 sows were taken. All sows originated from farms where reproductive disorders appeared. In general, 1069 samples were collected. Two enrichment methods were used in this study; fast enrichment techniques in ITC broth, then plating onto CIN agar (ITC/CIN), and cold enrichment in phosphate buffered saline followed by plating onto CIN agar (PBS/CIN). From all samples examined, 96 Y. enterocolitica strains were isolated including 57 (59.4%) from rectal swabs of sows, followed by 6 (6.3%) from vaginal swabs and 2 (2.1%) from placentas. The bacteria were isolated from tissues of 18 out of 97 aborted fetuses. A total of 60 strains were selected for further examination--29 strains originated from aborting sows and 31 from aborted fetuses. Among strains examined 54 isolates (90%) belonged to the biotype 1A of Y. enterocolitica and to the different serotypes O:3, O:5, O:6, O:7/13, O:8 and NT (not typable). Only 6 strains belonged to serotype O:3, biotype 4 Y. enterocolitica. Our study has revealed the possibility of Y enterocolitica isolation from internal organs of aborted swine fetuses and sows from farms with reproductive disturbances. The results suggest the connection between fetal death, pregnancy course disorders and Y. enterocolitica infection.
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PMID:Isolation of Yersinia enterocolitica from aborted fetuses and sows in pig farms with reproductive disturbances. 1964 48