Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UNIPROT:P30536 (PBS)
 9,886 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

We have studied the induction of immune tolerance by exogenous exposure to schistosomal egg antigen (SEA). We compared routes of administration, exposure to different forms of SEA (eggs, normal SEA, reultracentrifuged SEA), and treatment schedules. Tolerance was evaluated in comparison to SEA sensitization, after challenge with SEA or eggs. Anti-SEA responses were measured by ELISA assays, and cell-mediated responsiveness by 24-hr skin tests and pulmonary granuloma formation 8 and 16 days after intravenous egg injection. Oral administration of either SEA in PBS or NaHCO3, or eggs in PBS did not induce tolerance or sensitization. Reultracentrifuged SEA was similar to SEA in inducing cell-mediated immune response. Intrathymic exposure to SEA either had no effect or sensitized the animal for subsequent exposure to SEA. Two intraperitoneal injections of 0.5 to 1.0 mg of SEA lead to reduced cell-mediated responses, but resulted in high levels of anti-SEA antibody, suggesting a state of split tolerance or immune deviation. Our studies suggest that with complex, poorly defined antigens such as SEA, many of the classic methods of eliciting tolerance fail. The biologic significance of the form of tolerance inducible by high doses of SEA remains to be defined.
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PMID:Studies of tolerance in schistosomiasis. 375 55

The regulatory functions of IL-12 on granuloma formation by the eggs of S. japonicum in the lungs of BALB/c mice were studied. Mice were injected i.v. with the eggs to induce the pulmonary granuloma. On days 0, 1, 2, 3, 7, 8 after injection, mice were injected i.p. with murine rIL-12 at a dose of 1 microgram day-1 (Group I) or 3 micrograms day-1 (Group II) or with PBS as a control (Group III). On day 10, all mice were sacrificed. The areas of pulmonary granulomas in histological sections were quantitatively measured by image analyser and the levels of mRNA for IL-10, IFN-gamma, IL-12 and IL-5 were assayed by reverse transcriptase PCR (RT-PCR). The results showed that the mean area per granuloma was significantly decreased in Groups I and II as compared with Group III. As for cytokine induction, IL-10- and IFN-gamma-specific bands appeared in Groups I and II, but not in Group III. Bands for IL-5 appeared in all groups, while bands for IL-12 were not detected in any group. It is suggested that IL-12 could inhibit the Th2 dominant granuloma formation induced by the eggs of S. japonicum.
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PMID:Effect of IL-12 on granuloma formation induced by injected Schistosoma japonicum eggs. 927 91