Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UNIPROT:P30536 (PBS)
 9,886 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

The present paper summarizes the experience of the authors in the setting up of the radioimmunoassay (RIA) for human follicle simulating hormone (H-FSH), with the purified antigen for radioiodination, the F-FSH standard and the specific antibodies kindly donated by the National Pituitary Agency of the National Institutes for Arthritis, Metabolic and Digestive Diseases of the National Institutes of Health, Bethesda, MD. U.S.A. The conditions for the RIA have modified somewhat and simplified with respect to the suggested instructions accompanying the reagents. Thus, the amount of Chloramine T and the time of exposure of the labeled H-FSH (H-FSH) has been studied. It is always purified on Sephadex G-100 immediately before addition to the RIA and in this manner it may be used for up to 2 month after labeling when kept at --20 degrees C. Curves obtained at different dilutions of the H-FSH Standard, carried out with phosphosaline buffer, pH 7.4-7.8 (PBS) containing 1 % human serum albumin, or with horse plasma, of with PBS containing 0,25 % serum from non-immune rabbits (RIA Buffer) have been compared iwth those abtained by serial dilutions of sera from post-menopausal with these diluents. The most consistent results were obtained with the RIA buffer as diluent. The redisual error was smaller, and serial of dilution curves of the H-FSH standard were parallel to those of plasma and acetone precipitates of urine from post-menopausal women. Parallelism was not god using those serum. Results using PBS contianing human seum albumin were poor. PBS containing bovine serum albumin was avoided as some batches were found to interfere with the binding of the F-FSH to the antibody. The stability of the different dilutions of the H-FSH standard prepared in RIA buffer was tested. It was found that the standard curves could be prepared, pipetted into the RIA tubes and kept ready, frozen at --20 degrees C for one to two months. This shortens the actual setting up of a given RIA and decreases interassay variation of results. Parallelism of the H-FSH standard curve with serial dilutions (in RIA-buffer) of sera from women on the day of the preovulatory was confirmed. The data obtained in men and women, during stimulation with LH-RH are also given. No cross reactivity was found the HCG or sera from women, in agreement with the fact that the antiserum had been absorbed with HCG. There is, however, a considerable degree of cross reactivity with H-TSH; Thus, sera containing 15 muU/ml H-TSH or more, would give false H-FSH results. Such H-TSH values are not only found in hypothyroid patients, but might be reached during TRH responses to TRH.
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PMID:[Validation of the radioimmunoassays for pituitary gonadotropins II. Human follicle stimulating hormone (author's transl)]. 446 72

In the present work the effects of oestradiol benzoate (EB) on pituitary and plasma concentrations of TSH, plasma T4 and T3, and thyroidal activity of male and female rats have been studied. Wistar rats weighing between 150 to 200 g were injected sc with varying doses of EB in corn oil for 9 or 30 days. The animals were exsanguinated by cardiac puncture and the hypophyses removed and individually homogenized at 4 degrees C in 200 microliters PBS buffer. Pituitary and plasma TSH were measured by radioimmunoassay. Thyroidal activity was evaluated by a 4 h 131I uptake and by 48 h thyroidal release plasma slopes derived form the ratio PB[125I] (from thyroidal secretion) to PB[131I] (from exogenous [131I]T4). In both male and female rats the 10 and 25 micrograms doses of EB produced a significant decrease in pituitary TSH content; this effect was more pronounced when the 25 micrograms dose was given over 30 days. Plasma T4 decreased significantly; plasma T3 was moderately elevated in all groups (NS) and significantly increased in female rats treated with 25 micrograms EB (P less than 0.01). It is concluded that EB induced a marked depression of intrapituitary TSH, probably due to a decrease in synthesis, without affecting the release of TSH into the circulation. Moreover, EB accelerated peripheral T4 kinetics and thyroid gland activity, albeit to a moderate degree.
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PMID:Effects of oestradiol benzoate on the pituitary-thyroid axis of male and female rats. 682 63

The effect of immunoneutralization of gonadotropin-releasing hormone (GnRH) on LH secretion and concentrations of GnRH receptor, GnRH receptor mRNA, and gonadotropin subunit mRNA in pituitary tissue of orchidectomized sheep (wethers) was assessed. Thirty-six wethers were assigned at random to one of six treatment groups (six wethers per group). Thirty wethers (groups 2 to 6) received 200 ml, (i.v.) of anti-GnRH antisera at passive immunization (PI). Anterior pituitary tissue was collected .5, 1, 2, 4, or 8 d after PI from wethers in groups 2 to 6, respectively. Pituitary tissue was also collected from unimmunized wethers (Group 1). Intravenous administration of anti-GnRH sera increased anti-GnRH activity to 69.1 +/- 7% (percentage of total 125I-labeled GnRH bound by a 1:1,000 serum:GEL-PBS dilution) within 1 h of PI. Anti-GnRH activity declined gradually during the period after PI, and 8 d after PI anti-GnRH activity was 57.2 +/- 1.7%. Serum concentration of LH was significantly reduced, relative to the pretreatment (16.1 +/- 1.8 ng/mL) level, within 4 h (7.6 +/- 1.5 ng/mL) of PI, and the LH level was 10% of the pretreatment concentration 8 d after PI (1.6 +/- 0.2 ng/mL). Steady-state concentration of GnRH receptor mRNA decreased progressively during the period after PI and was significantly reduced, relative to the level in unimmunized control wethers (.44 +/- .03 pg/micron total RNA) d after PI. Tissue concentrations of GnRH receptor and mRNA for the alpha, LH alpha, and FISH beta subunits were also reduced (P < .05) by PI. These data indicate that maintenance of steady-state concentrations of GnRH receptor and GnRH receptor mRNA requires continued GnRH stimulation.
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PMID:Concentration of gonadotropin-releasing hormone receptor messenger ribonucleic acid in pituitary tissue of orchidectomized sheep: effect of passive immunization against gonadotropin-releasing hormone. 902 65

Pituitary adenylyl cyclase-activating polypeptide (PACAP) is a potent factor in the regulation of neurotransmission, neuroprotection, neurogenesis and anti-inflammation. We here examined the neuroprotective effect of PACAP on injury to the spinal cord tissue of adult rats, induced by dropping a 10 g NYU impactor from the height of 25 mm (moderate injury) or 50 mm (severe injury). PACAP was found to effectively attenuate cell apoptosis in the spinal cord with moderate injury. However, treatment with PACAP had a lesser effect on decreasing DNA fragmentation in the lesion center of the spinal cord with severe contusion injury. Yet, greater extended neural fibers and motor neurons were observed in the rostral and caudal regions of the PACAP-treated spinal cord when compared to that seen in the PBS-treated control. Our findings indicate the beneficial effect of PACAP for the treatment of spinal cord injury (SCI).
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PMID:Pituitary adenylate cyclase-activating polypeptide prevents cell death in the spinal cord with traumatic injury. 1591 92