Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: UNIPROT:P30536 (PBS)
 9,886 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Serpulina hyodysenteriae produces an oxygen-stable heat-labile hemolysin that may be an important virulence factor in the pathogenesis of swine dysentery. We examined the effect of Ca2+, Co2+, Cu2+, Fe2+, Mg2+, Mn2+, Ni2+, and Zn2+ on the hemolytic activity of cell-free supernatant (CFS) from S. hyodysenteriae, isolate B204. Cells harvested from late logarithmic phase cultures were incubated in phosphate-buffered saline containing glucose and RNA-core (PBS-GR) with or without cations and the hemolytic activity of CFS obtained after successive 30 min incubation and washing cycles was determined. The addition of either ZnSO4 or CuSO4 to the PBS-GR caused complete inhibition of hemolytic activity after 3 cycles; other cations gave results similar to control extracts. Reduction in the concentration of Zn2+ in CFS by 60 to 80% after each incubation cycle and binding of Zn2+ by EDTA indicated that Zn2+ was associated with the cell fraction, and inhibition of hemolysin activity was specifically mediated by Zn2+. When the spirochetes were washed after incubation in the presence of ZnSO4 for 2 cycles and incubated in fresh PBS-GR without Zn2+, inhibition of hemolysin activity remained unchanged, indicating that the inhibitory effect of ZnSO4 was due to a direct action of ZnSO4 on the spirochetes. Since neither the viability of the spirochetes nor the activity of pre-formed hemolysin were affected by the presence of ZnSO4, the inhibitory effect of Zn2+ cations was attributed to reduced biosynthesis by viable S. hyodysenteriae cells rather than interference of Zn2+ cations with lysis of erythrocytes by the hemolysin. Transmission electron microscopic examination of spirochetes after incubation in PBS-GR containing ZnSO4 revealed clumping of ribosomes and clearing of cell cytoplasm.
 ...
PMID:Effect of divalent cations on hemolysin synthesis by Serpulina (Treponema) hyodysenteriae: inhibition induced by zinc and copper. 780 26

Seventeen Serpulina hyodysenteriae strains isolated from faeces, rectal swabs and intestinal contents of pigs with Swine Dysentery, from farms located in Buenos Aires province were serotyped. Samples on selective media (trypticase soy agar added by 5% ovine blood, 400 mg/l spectinomicin, 30 mg/l colistin, 30 mg/l vancomycin) were streaked and incubated under anaerobic atmosphere for 72 h at 42 degrees C. Suspected S. hyodysenteriae growth were identified by strong beta-hemolytic zone, without colonies, and the spirillar morphology, using the Victoria Blue 4-R stain were criteria following by S. hyodysenteriae preliminar identification. The following antigens were made by phenolic extraction from a concentrated inocula washed twice in PBS pH 7: whole-cell (WC), boiled cell (BC) and lipopolysaccharide (LPS). Two serological test were: coagglutination and immunodiffusion, using polyclonal rabbit antisera against the 9 serotypes of S. hyodysenteriae and S. innocens, using WC and BC like antigens for the first test and BC and LPS for the second. The Dot-ELISA Test was performed using BC and LPS antigens and monoclonal antibodies (AbM) against serotypes 1, 2, 3, 8, 9 of S. hyodysenteriae, AbM species-specific and AbM against S. innocens. All isolated S. hyodysenteriae strains belonged to serotype 8. Like in other countries occurred, it would exit a high regional prevalence of S. hyodysenteriae serotype, being the serotype 8 in Argentine.
 ...
PMID:[Serotyping of strains of Serpulina hyosenteriae isolated form swine with porcine dysentery symptoms in the province of Buenos Aires]. 1093 52