Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UNIPROT:P30536 (PBS)
 9,886 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

One-hundred-eight stool samples, collected in a fishing village of Senegal from 72 apparently healthy subjects and from 36 patients with gastrointestinal disorders, were examined for the presence of Y. enterocolitica. After 1, 2, 3 weeks of cold enrichment with PBS 1/15M, pH 7.6, plating was performed on MacConkey Agar after use of the alkali method. No Yersinia strains were isolated.
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PMID:Attempt of Yersinia enterocolitica isolation from human feces in Senegal. 369 53

We have studied the distribution of actin, using NBD-phallacidin as a probe, in isolated sheets of seminiferous epithelia and denuded tubule walls of the rat. Sheets of intact seminiferous epithelia were separated from tubule walls using EDTA in PBS. The isolated epithelia and denuded tubule walls were fixed, mounted on slides, made permeable with cold acetone (-20 degrees C), and then treated with NBD-phallacidin. Actin was observed in myoid cells, in ectoplasmic specializations of Sertoli cells, and in Sertoli cell regions adjacent to tubulobulbar processes of late spermatids. In myoid cells, filament bundles course in circular and longitudinal directions relative to the tubule wall. In Sertoli cells viewed at an angle perpendicular to the epithelial base, actin filaments in ectoplasmic specializations adjacent to junctional complexes circumscribe the bases of the cells. Filament bundles in ectoplasmic specializations adjacent to germ cells closely follow the contour of and are arranged parallel to the long axis of the developing acrosome. Sertoli cell regions adjacent to tubulobulbar processes of late spermatids stain intensely with NBD-phallacidin. Isolated seminiferous epithelia, combined with NBD-phallacidin as a probe for actin, provide an ideal model system in which to study further the contractile properties of Sertoli cell ectoplasmic specializations and the possible involvement of these structures in events that occur during spermatogenesis.
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PMID:Distribution of actin in isolated seminiferous epithelia and denuded tubule walls of the rat. 407 62

Kinetics of pinocytosis of FITC-Dextran (mw 70 000) was analysed in mouse L-cells using flow cytometry with a fluorescence-activated cell sorter (FACS). In each experiment information on 2 X 10(4) individual, living cells was obtained. The population of L-cells thus analysed was shown to be rather homogeneous with respect to cell size, and the peak of the FITC-fluorescence curve--a histogram of the accumulated signals from L-cells exposed to FITC-Dextran--was shown to be representative of the average size of the L-cells. It was found that the cellular accumulation of FITC-Dextran was proportional to the tracer. However, the rate of accumulation decreased with increasing time of incubation (up to 3 h). When cells were pulse-labeled for 10 min at 37 degrees C, rinsed carefully with ice-cold PBS, and reincubated at 37 degrees C for various periods of time, about 50% of the initial cellular FITC-fluorescence disappeared within approximately 15 min of reincubation, whereafter no measurable decrease in cellular fluorescence was seen. In addition, a rapid increase of intact FITC-Dextran molecules in the reincubation medium occurred within the first 15 to 30 min of reincubation, whereafter no further increase took place. Thus, a large portion of previously endocytosed FITC-Dextran becomes rapidly exocytosed, while the rest becomes sequestered within a compartment from where little or no exocytosis occurs. The deviation from linearity in accumulation related to time, and the distinct biphasic kinetics of exocytosis are taken to support a generalized two-compartment model for endocytosis and membrane recycling, the two compartments being endocytic vacuoles (endosomes) and (secondary) lysosomes, respectively.
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PMID:Kinetics of pinocytosis studied by flow cytometry. 620 51

Mouse blastocysts in delay of implantation and after an 18-hour activation for implantation by estrogen were recovered by flushing with glutaraldehyde containing Alcian Blue or by flushing with cold Dulbecco's PBS containing 0.1% sodium azide for further processing according to an Alcian Blue technique and a ConA-latex technique, respectively. Blastocysts in delay of implantation showed no or only faint staining with Alcian Blue, while blastocysts activated for 18 hours displayed a marked staining of the abembryonic pole. Binding of ConA-latex spheres demonstrated a markedly higher density at the abembryonic end of both delayed and implanting blastocysts. It is concluded that, as demonstrated by the Alcian Blue technique, the abembryonic trophoblast, which is the first one to attach and invade at implantation, has changed the properties of the extracellular coat, probably in a way that favors increase of adhesiveness and invasiveness. The similarity in pattern of ConA binding of both delayed and implanting blastocysts, however, suggests that this property is related more to preimplantational differences in proliferative activity of the two poles than to implantatory changes.
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PMID:Polar differences of delayed and implanting mouse blastocysts in binding of Alcian Blue and concanavalin A. 746 84

In order to answer the question of whether there is an optimal buffer system for the preservation and reoxygenation period in liver transplantation, sodium/potassium phosphate, HEPES, TRIS (THAM), MOPS and histidine/His-HCl buffers were investigated. The buffers were added to an "extracellular" electrolyte composition of preservation solution. The solutions were incubated with in vitro cultures of pig hepatocytes in two different models. I: during cold hypoxia (4 degrees C, PO2 < 0.1 mmHg) for 24 h, and II. during the reoxygenation period of 3 h after preservation in UW solution. Cell viability, cell detachment rate, and LDH and GOT liberation were used as parameters of cell alteration. The lowest amount of enzyme release during the preservation period and reoxygenation was obtained using sodium or potassium phosphate buffer. Rising LDH and GOT liberation rates during preservation and reoxygenation were observed with HEPES and TRIS buffer. The enzyme release induced by these three buffer systems correlated with their pKa values. Higher pH of the preservation solution resulted in higher enzyme leakage from the cells. In contrast, the Histidine/HCl buffer system with low pH led to striking cell damage during preservation as well as during reoxygenation. MOPS, a weak acid with the lowest pH in solution, led to the lowest enzyme release during the preservation period, but to high enzyme release after reoxygenation with standard medium. Incubation of the cultures with MOPS after UW preservation resulted in lower enzyme levels in comparison to the controls. In summary, PBS had the best results in our study.
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PMID:[The effect of buffers in liver preservation solutions on hepatocytes in a model of in vitro preservation and reoxygenation]. 799 Jun 20

The aim of the study was to delineate the most optimal preservation conditions for small bowel grafts. Established preservation solutions such as EuroCollins, University of Wisconsin, histidine-tryptophane-ketoglutarate-Brettschneider, phosphate-buffered sucrose (PBS 140), and 3 new solutions--extracellular fluid (ECF), lactobionate fructose, and a modified lactobionate fructose solution--were compared with saline to determine the most optimal solution for the intestine. Recipient survival, standard histology, and glutaminase activity were used to assess the degree of injury encountered after 12 hr of preservation followed by transplantation. To evaluate the various preservation conditions, ECF was used at pH 6.8 (original ECF). Grafts were preserved most optimally when a vascular washout after the cold storage period was omitted and when topical rewarming of the graft with 37 degrees C saline before reperfusion was performed. Graft survival was not significantly different after preservation with any solution tested (50-83%). Highest graft survival (83%) was achieved with lactobionate fructose and PBS140. Histologic evaluation 20 min after reperfusion revealed minor differences between most groups; a slight advantage was observed for PBS140-preserved grafts. Mucosal glutaminase activity of PBS140-preserved grafts was significantly higher 20 min after reperfusion compared with any other solution evaluated, indicating a superior graft function. These data indicate that different preservation conditions have a great impact on postoperative graft survival and that PBS140 might be preferable to any of the other preservation solutions tested.
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PMID:Evaluation of preservation conditions and various solutions for small bowel preservation. 814 Jun 26

The placenta is a rich source of immunocompetent cells. We have studied the phenotype, number and origin of placental mononuclear blood cells isolated from 32 normal term placentae using 4 color flow cytometry. Respective maternal and cord blood leucocyte preparations were also compared. Placental tissue without extraembryonic membranes was cut into small pieces and divided. One portion was washed extensively with ice-cold PBS. Both tissue portions were disrupted in a blender and cells were dissociated by using a 180 mu sieve. Leucocytes were isolated by Ficoll-Hypaque density gradient centrifugation. Maternal and cord bloods were HLA typed and in cases of HLA-A2 or B7/40 disparity, monoclonal anti-HLA antibodies to these antigens showed that unwashed placental tissue contained 35% maternal and 65% fetal cells. This ratio, however, was not reflected for a given cell phenotype. In comparison, washed placental tissue contained cells of fetal origin only. Both unwashed and washed placental tissue contained fewer CD3 and CD4, but more CD8 cells than maternal and cord blood. Markers of NK cells such as, CD16, CD56, and CD57 showed this cellular phenotype to be 15 times more abundant in the placental preparations than in cord and maternal blood. The quantitative differences between peripheral blood and placental CD8 and NK cells were further explored with an antiprogesterone receptor antibody in combination with anti-CD8, anti-CD57 and anti-HLA-DR. The number of progesterone receptor (PGR) positive cells was three times higher in placental tissues than in cord or maternal blood. These data indicate that the phenotypic frequencies of certain placental leucocytes are significantly different from maternal and fetal peripheral blood. Progesterone and the presence of PGR may be important in the differential retention of placental leucocytes.
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PMID:Phenotypic characterization of normal human placental mononuclear cells. 827 Dec 37

Helicobacter pylori cells cultured on solid medium were quantitatively tested for haemolytic activity against erythrocytes of man, sheep, the guinea pig and rabbit. Using 4-day and 8-day cultures of two standard strains (ATCC 43504, IMMi 676), human erythrocytes were not lysed by 10% bacterial suspensions. Rabbit erythrocytes were the most sensitive to 8-day cultures. Hot-cold incubation yielded the highest haemolysis titres. The extent of haemolysis strongly correlated with the number of bacterial cells. Supplementation of the test medium (PBS, pH 7.4) with L-cysteine, dithiothreitol, MgCl2, EDTA, cholesterol, lecithin or sphingomyelin did not influence the haemolysis titres. They were significantly reduced in the presence of pronase E, human serum, bovine serum albumin or CaCl2, and by heat treatment of the bacteria. Supplementation of the test medium with cardiolipin strongly increased the haemolysis titres. Comparing the cell-associated haemolytic activity of 18 strains, the titres ranged from < 2 to 64, with a median titre of 16. No correlation was found between the haemolytic activity and phospholipase C activity of the cell suspensions. It was concluded that the formation of lysophosphatides and non-enzymatic factors rather than a sulphydryl-activated cytolysin or phospholipase C are responsible for the cell-associated haemolytic activity. This property may be involved in the pathogenicity and virulence of Helicobacter pylori.
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PMID:Cell-associated haemolytic activity of Helicobacter pylori. 850 Apr 89

Yersinia spp. was examined in three rivers and two lakes located in the Province of San Luis, Argentina, over a 1-year period. Water samples were concentrated either by Moore's gauze technique or by filtering through diatomaceous earth. Five enrichment media: yeast extract--Bengal rose broth (YER) with bile-oxalate-sorbose broth (BOS); 67 mmol/L phosphate-buffered saline (pH 7.6; PBS); PBS enriched with a 1% mannitol and 1% peptone (PBSMP); PBS with lyzed 0.5% sheep blood (PBSB); Wauters broth (W); and five plating media: Mac Conkey agar (MC); Salmonella--Shigella agar (SS); 5% sheep blood agar (BA); lactose-sucrose-urea agar (LSU) and irgasan-novobiocin agar (IN) were used. The following strains were isolated: Y. intermedia B1 O:4,32-4,33 Lis Xz (four strains), Y. intermedia B1 O:57 Lis Xo (one strain), Y. intermedia B2 O:57 Lis Xo (one strain), Y. enterocolitica B1 O:10-34 Lis Xz (one strain), and Y. frederiksenii undetermined biovar, O:16-16,29 Lis Xz (two strains). The incidence of isolation of Yersinia spp. was 7.14%. YER-BOS proved to be the best enrichment method since it allowed the highest recovering Yersinia spp. strains. Among plating media, the best results were obtained with MC. Apparently, the isolation of Yersinia spp. can be related to environmental variables such as temperature differences between cold and warm seasons. Negative results obtained during virulence assays suggest that isolated strains lack the pathogenic potential against man.
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PMID:Yersinia ssp. in surface water in San Luis, Argentina. 854 92

Four experiments determined the kinetics of in vitro maturation and fertilization of cat oocytes and the effects of prolonged cold storage of ovaries before oocyte recovery on in vitro maturation/in vitro fertilization (IVM/IVF) success. Domestic cat ovaries were collected at ovariohysterectomy and stored at 4 degrees C in PBS until oocyte recovery and culture in Eagle's minimal essential medium (EMEM) containing FSH, LH, oestradiol and BSA for maturation. In Expt 1, meiotic maturation was assessed at 0, 12, 24, 38 and 48 h of culture. After 24 h, > 61% of oocytes were in telophase I or metaphase II. In Expt 2, oocytes were recovered from ovaries stored for 24, 48 or 72 h and cultured in EMEM for 24 h. There was no difference among groups (P > 0.05) in the ability to achieve nuclear maturation (mean +/- SEM, 57.1 +/- 5.3%, 60.4 +/- 5.4%, 55.4 +/- 15.1% for 24, 48 and 72 h, respectively). Fertilization and embryo development after insemination at 16, 24, 32, 40 and 48 h of culture were examined in Expt 3. Of 98 oocytes inseminated at 32 h, 69% cleaved, 59% developed into morulae and 13% into blastocysts, more (P < 0.05) than those oocytes inseminated at earlier and later times. Development to blastocysts occurred after insemination at 16 (1.2%), 24 (9.1%) and 32 (13.3%) h of culture, but not after insemination at 40 or 48 h. Expt 4 involved cold storage of ovaries for 24, 48 or 72 h before oocyte recovery and insemination at 32 h of culture (the optimal time measured in Expt 3). Compared with storage for 24 h, fertilization success was lower (P < 0.05) in the 48 and 72 h groups, and, although 9.1% of inseminated oocytes from the 24 h storage group developed to blastocysts, none (P < 0.05) achieved this stage after 48 or 72 h of storage. These results indicate that domestic cat oocytes reach nuclear maturity by 24 h in culture and can be fertilized and develop to blastocysts optimally after insemination at 32 h. Oocytes recovered from ovaries stored at 4 degrees C for up to 72 h are capable of reaching telophase I or metaphase II in vitro. However, only oocytes stored within the ovary for 24 h produce blastocysts, indicating that the ability to achieve nuclear maturation is an inadequate indicator of fertilization and developmental competence.
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PMID:Development to blastocysts of domestic cat oocytes matured and fertilized in vitro after prolonged cold storage. 866 38


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