Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: UNIPROT:P30044 (
antioxidant enzyme
)
8,037
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
DNMT2
is a DNA/tRNA cytosine methyltransferase that is highly conserved in structure and function in eukaryotes. In plants however, limited information is available on the function of this methyltransferase. We have previously reported that in the moss
Physcomitrella patens
,
DNMT2
plays a crucial role in stress recovery and tRNA
Asp
transcription/stability under salt stress. To further investigate the role of
PpDNMT2
at genome level, in this study we have performed RNA sequencing of
ppdnmt2
. Transcriptome analysis reveals a number of genes and pathways to function differentially and suggests a close link between
PpDNMT2
function and osmotic and ionic stress tolerance. We propose
PpDNMT2
to play a pivotal role in regulating salt tolerance by affecting molecular networks involved in stress perception and signal transduction that underlie maintenance of ion homeostasis in cells. We also examined interactome of PpDNMT2 using affinity purification (AP) coupled to mass spectrometry (AP-MS). Quantitative proteomic analysis reveals several chloroplast proteins involved in light reactions and carbon assimilation and proteins involved in stress response and some not implicated in stress to co-immunoprecipitate with PpDNMT2. Comparison between transcriptome and interactome datasets has revealed novel association between
PpDNMT2
activity and the
antioxidant enzyme
Superoxide dismutase (SOD), protein turnover mediated by the Ubiquitin-proteasome system and epigenetic gene regulation. PpDNMT2 possibly exists in complex with CuZn-SODs
in vivo
and the two proteins also directly interact in the yeast nucleus as observed by yeast two-hybrid assay. Taken together, the work presented in this study sheds light on diverse roles of
PpDNMT2
in maintaining molecular and physiological homeostasis in
P. patens
. This is a first report describing transcriptome and interactome of
DNMT2
in any land plant.
...
PMID:Transcriptome Analysis of
ppdnmt2
and Identification of Superoxide Dismutase as a Novel Interactor of DNMT2 in the Moss
Physcomitrella patens
. 3284 34
