Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
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Drug
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Compound
Target Concepts:
Gene/Protein
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Query: UNIPROT:P30044 (
antioxidant enzyme
)
8,037
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Large mafs are transcriptional factors and members of the basic
leucine zipper
(b-Zip) superfamily. Since we previously identified expression of c-maf in mouse kidney, we presently investigated the mRNA expression profile in the kidney of c-maf gene knockout mice by using DNA microarray, and plasma glutathione peroxidase-3 (GPx3) was predominantly downregulated. We focused on the relation between the expression level of c-maf and GPx3 in vivo and in vitro. Since GPx3 is an
antioxidant enzyme
, oxidative stress was induced by exposing a culture cell derived from mouse renal tubules (mIMCD3) to hydrogen peroxide. Real-time PCR demonstrated that mRNA expression of both c-maf and GPx3 increased in parallel during exposure to oxidative stress in a time- and dose-dependent manner. Then, the mIMCD3 cells were transfected with c-maf-cDNA containing plasmid, which resulted in an increase in mRNA and protein expression of GPx3 compared with the control cells. Thus, c-maf may be transcriptional regulator of GPx3 expression and modulate the antioxidative pathway in the kidney.
...
PMID:Correlation between the expression level of c-maf and glutathione peroxidase-3 in c-maf -/- mice kidney and c-maf overexpressed renal tubular cells. 1689 Jan 89
To understand the link between long-term drought tolerance and short-term drought responses in plants, transgenic rice (Oryza sativa L.) plants over-expressing the maize C
4-
pepc gene encoding phosphoenolpyruvate carboxylase (PC) and wild-type (WT) rice plants were subjected to PEG 6000 treatments to simulate drought stress. Compared with WT, PC had the higher survival rate and net photosynthetic rate after 16days of drought treatment, and had higher relative water content in leaves after 2h of drought treatment as well, conferring drought tolerance. WT accumulated higher amounts of malondialdehyde, superoxide radicals, and H
2
O
2
than PC under the 2-h PEG 6000 treatment, indicating greater damages in WT. Results from pretreatments with a Ca
2+
chelator and/or antagonist showed that the regulation of the early drought response in PC was Ca
2+
-dependent. The NO and H
2
O
2
levels in PC lines were also up-regulated via Ca
2+
signals, indicating that Ca
2+
in PC lines also reacted upstream of NO and H
2
O
2
. 2-h drought treatment increased the transcripts of CPK9 and CPK4 in PC via positive up-regulation of Ca
2+
. The transcripts of NAC6 [NACs (NAM, ATAF1, ATAF2, and CUC2)] and bZIP60 (basic
leucine zipper
, bZIP) were up-regulated, but those of DREB2B (dehydration-responsive element-binding protein, DREB) were down-regulated, both via Ca
2+
signals in PC. PEPC activity, expressions of C
4
-pepc, and the
antioxidant enzyme
activities in PC lines were up-regulated via Ca
2+
. These results indicated that Ca
2+
signals in PC lines can up-regulate the NAC6 and bZIP60 and the downstream targets for early drought responses, conferring drought tolerance for the long term.
...
PMID:Improved short-term drought response of transgenic rice over-expressing maize C
4
phosphoenolpyruvate carboxylase via calcium signal cascade. 2888 62
