Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: UNIPROT:P30044 (
antioxidant enzyme
)
8,037
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
In plant cells, vacuolar H
+
-ATPases (V-ATPases) are responsible for deacidification of the cytosol and energisation of the secondary transport processes across the tonoplast. A number of V-ATPase subunit genes have been demonstrated to be involved in the regulation of the plant response to water deficit. However, there are no reports on the role of
V-ATPase subunit A
(VHA-A) in dehydration tolerance of cotton. In this study, cotton
GhVHA-A
gene was functionally characterized, especially with regard to its role in dehydration stress tolerance. Expression analysis showed that
GhVHA-A
was differentially expressed in various cotton organs and was induced by dehydration, low temperature, high salinity, and abscisic acid treatment in leaves. We also report that
GhVHA-A
improve dehydration tolerance in transgenic tobacco and cotton. Virus-induced gene silencing of
GhVHA-A
decreased the tolerance of cotton plantlets to dehydration stress. Silencing
GhVHA-A
decreased chlorophyll content and
antioxidant enzyme
activities and increased malondialdehyde (MDA) content in cotton under dehydration stress. However, transgenic tobacco expressing
GhVHA-A
exhibited enhanced dehydration resistance, resulting in reduced leaf water loss, higher average root length, and lower MDA levels under dehydration stress. Meanwhile, overexpression of
GhVHA-A
in tobacco conferred water deficit tolerance by enhancing osmotic adjustment (proline) and the activities of the antioxidant enzymes superoxide dismutase and peroxidase, thereby enhancing reactive oxygen species detoxification. These results suggest that
GhVHA-A
plays an important role in conferring resistance to dehydration stress. Our results have identified
GhVHA-A
as a candidate gene for improving dehydration tolerance in plants.
...
PMID:The Gene Encoding Subunit A of the Vacuolar H
+
-ATPase From Cotton Plays an Important Role in Conferring Tolerance to Water Deficit. 2993 May 64
