Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UNIPROT:P30044 (antioxidant enzyme)
 8,037 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Reactive oxygen species are important mediators of injury in acute renal failure (ARF). Although polymorphisms that affect key pro- and antioxidant enzymes might alter the susceptibility to oxidative stress-mediated injury, the use of genetic epidemiology for the study of oxidative stress-related genes has received little attention in ARF. The relationship of single-nucleotide polymorphisms in the coding region (C to T substitution at position +242) of the pro-oxidant enzyme NADPH oxidase p22phox subunit gene and in the promoter region (C to T substitution at position -262) of the antioxidant enzyme catalase gene to adverse clinical outcomes was evaluated prospectively in a cohort of 200 hospitalized patients with established ARF of mixed cause and severity. Genomic DNA was extracted from peripheral blood leukocytes and analyzed with a restriction fragment length polymorphism PCR method. Genotype-phenotype associations were characterized by measuring circulating nitrotyrosine and catalase activity. Observed and expected genotype frequencies were not significantly different, and overall baseline characteristics were not significantly different according to the various genotype groups. A genotype-phenotype association was demonstrable between the NADPH oxidase p22phox genotypes and plasma nitrotyrosine level (P = 0.06), as well as between the catalase genotypes and whole-blood catalase activity (P < 0.001). Compared with the NADPH oxidase p22phox CC genotype group, the T-allele group had a higher cumulative probability of remaining hospitalized (P = 0.03). Compared with the NADPH oxidase p22phox CC genotype, the T-allele carrier state was associated with 2.1-fold higher odds for dialysis requirement or hospital death (P = 0.01). This association persisted with 2.0- to 2.2-fold higher odds for this composite outcome after adjustment for race; gender; age; and the Acute Physiology and Chronic Health Evaluation II score (P = 0.03), the Multiple Organ Failure score (P = 0.01), or presence of sepsis (P = 0.02). The polymorphism in the gene that encodes the NADPH oxidase p22phox subunit at position +242 is associated with dialysis requirement or hospital death among patients with ARF. Larger studies are needed to confirm these relationships.
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PMID:NADPH oxidase p22phox and catalase gene variants are associated with biomarkers of oxidative stress and adverse outcomes in acute renal failure. 1718 82

Oxidative damage and iron redistribution are associated with the pathogenesis and progression of multiple sclerosis (MS), but these aspects are not entirely replicated in rodent experimental autoimmune encephalomyelitis (EAE) models. Here, we report that oxidative burst and injury as well as redistribution of iron are hallmarks of the MS-like pathology in the EAE model in the common marmoset. Active lesions in the marmoset EAE brain display increased expression of nicotinamide adenine dinucleotide phosphate (NADPH) oxidase (p22phox, p47phox, and gp91phox) and inducible nitric oxide synthase immunoreactivity within lesions with active inflammation and demyelination, coinciding with enhanced expression of mitochondrial heat-shock protein 70 and superoxide dismutase 1 and 2. The EAE lesion-associated liberation of iron (due to loss of iron-containing myelin) was associated with altered expression of the iron metabolic markers FtH1, lactoferrin, hephaestin, and ceruloplasmin. The enhanced expression of oxidative damage markers in inflammatory lesions indicates that the enhanced antioxidant enzyme expression could not counteract reactive oxygen and nitrogen species-induced cellular damage, as is also observed in MS brains. This study demonstrates that oxidative injury and aberrant iron distribution are prominent pathological hallmarks of marmoset EAE thus making this model suitable for therapeutic intervention studies aimed at reducing oxidative stress and associated iron dysmetabolism.
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PMID:Oxidative Injury and Iron Redistribution Are Pathological Hallmarks of Marmoset Experimental Autoimmune Encephalomyelitis. 2850 83

The crude protein hydrolysates of wild hazel have good immunoregulation and antioxidation effects. However, the components responsible for their antioxidation effect remain unknown. In this study, six antioxidative peptides (EW, DWDPK, ADGF, SGAF, ETTL, and AGGF) were tested for their protective effects on oxidative stress injury in human umbilical vein endothelial cells (HUVECs). The results demonstrated that the six peptides are nontoxic and have a protective effect on oxidative stress injury induced by Ang II. Three peptides (EW, ADGF, and DWDPK) inhibited the morphological changes, downregulated the content of lactate dehydrogenase and malondialdehyde, upregulated the activity of antioxidant enzymes catalase, total superoxide dismutase and glutathione peroxidase, and scavenged reactive oxygen species (ROS) in HUVECs. Quantitative reverse transcriptive polymerase chain reaction and western blot assays indicated that these three peptides regulated NADPH oxidase activity and ROS production by reducing NOX4 and p22phox levels. Overall, they have a significant protective effect against oxidative stress injury and have potential application in developing new functional foods. PRACTICAL APPLICATIONS: Corylus heterophylla Fisch is a good quality wild hazel distributed in Northeast China. Wild hazelnut of the species C. heterophylla Fisch was selected as experimental object and has high nutritive values and have abundant proteins (20%-30%), fats (40%-50%), carbohydrates (13%-24%), dietary fibers (8.2%-9.6%), vitamins, and micronutrients. Our results indicate that hazelnut peptides (EW, ADGF, and DWDPK) can ensure normal growth of cells by protecting important antioxidant enzyme systems, by enhancing antioxidant defense, by directly affecting nicotinamide adenine dinucleotide phosphate (NADPH) oxidase activity, and by reducing intracellular reactive oxygen species (ROS) production in HUVECs, indicating that the three antioxidative peptides have a protective effect against Ang II-induced oxidative stress injury. Therefore, the antioxidative peptides from C. heterophylla Fisch may be a promising candidate for functional food ingredients and/or pharmaceuticals.
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PMID:Protective role of hazelnut peptides on oxidative stress injury in human umbilical vein endothelial cells. 3135 65