Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UNIPROT:P30044 (antioxidant enzyme)
 8,037 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Quinone reductase (QR) is an oxidative-related gene and few studies have focused on its roles concerning salt stress tolerance in plants. In this study, we cloned and analyzed the QR gene from Salix matsudana, a willow with tolerance of moderate salinity. The 612-bp cDNA corresponding to SmQR encodes 203 amino acids. Expression of SmQR in Escherichia coli cells enhanced their tolerance under salt stress. In addition, transgenic Arabidopsis thaliana lines overexpressing SmQR exhibited higher salt tolerance as compared with WT, with higher QR activity and antioxidant enzyme activity as well as higher chlorophyll content, lower methane dicarboxylic aldehyde (MDA) content and electric conductivity under salt stress. Nitro blue tetrazolium (NBT) and 3,3'-diaminobenzidine (DAB) staining also indicated that the transgenic plants accumulated less reactive oxygen species compared to WT when exposed to salt stress. Overall, our results suggested that SmQR plays a significant role in salt tolerance and that this gene may be useful for biotechnological development of plants with improved tolerance of salinity.
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PMID:Overexpression of quinone reductase from Salix matsudana Koidz enhances salt tolerance in transgenic Arabidopsis thaliana. 2654 Oct 63

We used a willow species (Salix miyabeana cultivar SX64) to examine the differential secondary-effects of glyphosate and aminomethylphosphonic acid (AMPA), the principal glyphosate by-product, on chlorophyll metabolism and photosynthesis. Willow plants were treated with different concentrations of glyphosate (equivalent to 0, 1.4, 2.1 and 2.8kgha(-1)) and AMPA (equivalent to 0, 0.28, 1.4 and 2.8kgha(-1)) and evaluations of pigment contents, chlorophyll fluorescence, and oxidative stress markers (hydrogen peroxide content and antioxidant enzyme activities) in leaves were performed after 12h of exposure. We observed that AMPA and glyphosate trigger different mechanisms leading to decreases in chlorophyll content and photosynthesis rates in willow plants. Both chemicals induced ROS accumulation in willow leaves although only glyphosate-induced oxidative damage through lipid peroxidation. By disturbing chlorophyll biosynthesis, AMPA induced decreases in chlorophyll contents, with consequent effects on photosynthesis. With glyphosate, ROS increases were higher than the ROS-sensitive threshold, provoking chlorophyll degradation (as seen by pheophytin accumulation) and invariable decreases in photosynthesis. Peroxide accumulation in both AMPA and glyphosate-treated plants was due to the inhibition of antioxidant enzyme activities. The different effects of glyphosate on chlorophyll contents and photosynthesis as described in the literature may be due to various glyphosate:AMPA ratios in those plants.
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PMID:Differential effects of glyphosate and aminomethylphosphonic acid (AMPA) on photosynthesis and chlorophyll metabolism in willow plants. 2715 86

Receptor-like cytoplasmic kinases (RLCKs) play critical roles in biotic and abiotic stress responses in plants. However, the functions of RLCKs from the desert shrub willow Salix psammophila have not been characterized. Here, we focused on the biological function of SpRLCK1, which was previously identified as a potential drought-related gene. Phylogenetic analysis and subcellular localization revealed that SpRLCK1 was a cytoplasmic-localized protein with a protein kinase domain and belonged to the RLCK VIIa subclass. Gene expression profile revealed that SpRLCK1 was predominantly expressed in the root, being consistent with the GUS staining of pSpRLCK1:GUS transgenic plants. Additionally, the expression of SpRLCK1 was significantly induced by drought and salt stresses. To verify the function of SpRLCK1, we generated its overexpressing transgenic lines in Arabidopsis thaliana. The SpRLCK1-overexpressing plants exhibited higher tolerance to drought and salt stresses, as evidenced by the higher survival rate, relative water content and antioxidant enzyme activity than those of wild-type plants. The SpRLCK1-overexpressing plants enhanced drought and salt tolerance by improving ROS-scavenging activities. A co-expression network for SpRLCK1 was constructed, and the expression analysis indicated that SpRLCK1 regulated the expression of a series of stress-related genes. Taken together, our results demonstrate that SpRLCK1 confers plant drought and salt tolerance through enhancing the activity of antioxidant enzymes and cooperating with stress-related genes.
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PMID:The Salix psammophila SpRLCK1 involved in drought and salt tolerance. 3158 22

Phytoremediation assisted by plant growth-promoting bacteria (PGPB) is considered an effective strategy for cadmium (Cd) removal in contaminated sites. This study uses a hydroponic experiment to investigate how Sphingobium yanoikuyae Sy310 affects Cd accumulation capacity and tolerance of Salix matsudana Koidz (S. matsudana) roots. The results showed that Cd induced growth change and physiological response on S. matsudana roots, displaying with reduced root length, increased antioxidant enzyme activities, and most importantly, enhanced cell wall polysaccharide contents. The Sy310 inoculation enhanced Cd accumulation in roots and alleviated the Cd toxic effects by regulating root growth, antioxidant enzyme system, and cell wall polysaccharide remodeling. Under Cd stress, Sy310 significantly induced increased root length and biomass, as well as higher root IAA level and Cd retention in cell walls. The Sy310 inoculation enhanced root pectin and hemicellulose 1 content, and pectin methylesterase activity, indicating that more amount of -COOH and -OH in cell walls for binding Cd. With Sy310-regulated extensive Cd regional sequestration in root cell walls and enhanced catalase activity, the root H2O2 and malondialdehyde content decreased, which contributes to improve Cd tolerance of S. matsudana roots. Furthermore, the Sy310 inoculation did not affect root cell wall structure and oxidative stress in the absence of Cd, representing a well-symbiotic relationship between Sy310 and S. matsudana. Therefore, Sy310 plays an important role in expediting the phytoremediation process of Cd with S. matsudana and has practical application potential.
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PMID:Bacteria Sphingobium yanoikuyae Sy310 enhances accumulation capacity and tolerance of cadmium in Salix matsudana Koidz roots. 3222 21