Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: UNIPROT:P30044 (
antioxidant enzyme
)
8,037
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Extreme environmental conditions seriously affect crop growth and development, resulting in substantial reduction in yield and quality. However, chitin-binding proteins (CBP) family member
CaChiVI2
plays a crucial role in eliminating the impact of adverse environmental conditions, such as cold and salt stress. Here, for the first time it was discovered that
CaChiVI2
(Capana08g001237) gene of pepper (
Capsicum annuum
L.) had a role in resistance to heat stress and physiological processes. The full-length open-reading frame (ORF) of
CaChiVI2
(606-bp, encoding 201-amino acids), was cloned into TRV2:
CaChiVI2
vector for silencing. The
CaChiVI2
gene carries heat shock elements (
HSE
, AAAAAATTTC) in the upstream region, and thereby shows sensitivity to heat stress at the transcriptional level. The silencing effect of
CaChiVI2
in pepper resulted in increased susceptibility to heat and
Phytophthora capsici
infection. This was evident from the severe symptoms on leaves, the increase in superoxide (O
2
-
) and hydrogen peroxide (H
2
O
2
) accumulation, higher malondialdehyde (MDA), relative electrolyte leakage (REL) and lower proline contents compared with control plants. Furthermore, the transcript level of other resistance responsive genes was also altered. In addition, the
CaChiIV2
-overexpression in
Arabidopsis thaliana
showed mild heat and drought stress symptoms and increased transcript level of a defense-related gene (
AtHSA32
), indicating its role in the co-regulation network of the plant. The
CaChiVI2
-overexpressed plants also showed a decrease in MDA contents and an increase in
antioxidant enzyme
activity and proline accumulation. In conclusion, the results suggest that
CaChiVI2
gene plays a decisive role in heat and drought stress tolerance, as well as, provides resistance against
P. capsici
by reducing the accumulation of reactive oxygen species (ROS) and modulating the expression of defense-related genes. The outcomes obtained here suggest that further studies should be conducted on plants adaptation mechanisms in variable environments.
...
PMID:The
CaChiVI2
Gene of
Capsicum annuum
L. Confers Resistance Against Heat Stress and Infection of
Phytophthora capsici
. 3217 52
