Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: UNIPROT:P21817 (
RyR1
)
1,154
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Stromal interaction molecule 1 (STIM1) along with Orai1 mediates extracellular Ca
2+
entry into the cytosol through a store-operated Ca
2+
entry (SOCE) mechanism in various tissues including skeletal muscle. However, the role(s) of STIM2, a homolog of STIM1, in skeletal muscle has not been well addressed. The present study, first, was focused on searching for STIM2-binding proteins from among proteins mediating skeletal muscle functions. This study used a binding assay, quadrupole time-of-flight mass spectrometry, and co-immunoprecipitation assay with bona-fide STIM2- and SERCA1a-expressing rabbit skeletal muscle. The region for amino acids from 453 to 729 of STIM2 binds to sarcoplasmic/endoplasmic reticulum Ca
2+
-ATPase 1a (SERCA1a). Next,
oxalate
-supported
45
Ca
2+
-uptake experiments and various single-myotube Ca
2+
imaging experiments using STIM2-knockdown mouse primary skeletal myotubes have suggested that STIM2 attenuates SERCA1a activity during skeletal muscle contraction, which contributes to the intracellular Ca
2+
distribution between the cytosol and the SR at rest. In addition, STIM2 regulates Ca
2+
movement through
RyR1
during skeletal muscle contraction as well as SOCE. Therefore, via regulation of SERCA1a activity, STIM2 regulates both intracellular Ca
2+
distribution and Ca
2+
movement in skeletal muscle, which makes it both similar to, yet different from, STIM1.
...
PMID:STIM2 regulates both intracellular Ca
2+
distribution and Ca
2+
movement in skeletal myotubes. 2926 48
