Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: UNIPROT:P21817 (
RyR1
)
1,154
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
The ryanodine receptor type-I (
RyR1
) is one key player of the excitation-contraction coupling (E-CC) machinery. However,
RyR1
expression in human skeletal muscle disuse and plasticity changes are not well documented. We studied the expression and the functional modifications of
RyR1
following prolonged bed rest (BR) without and with exercise countermeasure (Resistive Vibration Exercise, RVE). Soleus biopsies were taken from a non-trained control (BR-CTRL) and trained (BR-RVE) group (each n = 10) before and after BR. In BR-
CTRL
group, a fibre type-specific immunopattern of
RyR1
(type-I < type-II) was documented, and
RyR1
immunofluorescence intensity and protein expression together with [(3)H]ryanodine binding were decreased after BR. In BR-RVE group,
RyR1
immunosignals were increased and fiber type specificity was no longer present.
RyR1
protein expression was unchanged, whereas [(3)H]ryanodine binding increased after BR. Confocal and biochemical analysis confirmed subcellular co-localisation and protein-protein interaction of
RyR1
with nitric oxide (NO)-synthase type-1 (NOS1). S-nitrosylation of
RyR1
was increased in BR-CTRLpost only, suggesting a reduction of
RyR1
open channel probability by nitrosylation mechanisms following prolonged disuse. We conclude that following extended body deconditioning in bed rest, RVE countermeasure maintained normal
RyR1
expression and nitrosylation patterns required for adequate E-CC in human performance control.
...
PMID:Ryanodine receptor type-1 (RyR1) expression and protein S-nitrosylation pattern in human soleus myofibres following bed rest and exercise countermeasure. 1828 81
