Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: UNIPROT:P21817 (
RyR1
)
1,154
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Ryanodine receptors (RyRs) are highly conductive intracellular Ca
2+
release channels and are widely expressed in many tissues, including the central nervous system. RyRs have been implicated in intracellular Ca
2+
overload which can drive secondary damage following traumatic injury to the spinal cord (
SCI
), but the spatiotemporal expression of the three isoforms of RyRs (
RyR1
-3) after
SCI
remains unknown. Here, we analyzed the gene and protein expression of RyR isoforms in the murine lumbar dorsal root ganglion (DRG) and the spinal cord lesion site at 1, 2 and 7 d after a mild contusion
SCI
. Quantitative RT PCR analysis revealed that RyR3 was significantly increased in lumbar DRGs and at the lesion site at 1 and 2 d post contusion compared to sham (laminectomy only) controls. Additionally, RyR2 expression was increased at 1 d post injury within the lesion site. RyR2 and -3 protein expression was localized to lumbar DRG neurons and their spinal projections within the lesion site acutely after
SCI
. In contrast,
RyR1
expression within the DRG and lesion site remained unaltered following trauma. Our study shows that
SCI
initiates acute differential expression of RyR isoforms in DRG and spinal cord.
...
PMID:Differential expression of ryanodine receptor isoforms after spinal cord injury. 2889 87
