Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UNIPROT:P20366 (substance P)
 21,176 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

The purpose of this study is to examine the role of peptidergic nerves (VIP, Substance P, Neurotensin) in Hirschsprung's disease (aganglionosis) and hypoganglionosis in relation to the normoganlionic state of the colon using a mechanographic technique in vitro. The following results were obtained. 1) Normoganglionic muscle strips demonstrated the presence of intact non-adrenergic inhibitory nerve. The activities of such nerves, however, were reduced in hypoganglionic muscle strips, and were absent in aganglionic muscle strips. 2) Peptidergic nerve activities by VIP, substance P, and Neurotensin were present in normoganglionic muscle strips, while they were reduced in hypoganglionic muscle strips, and absent in aganglionic muscle strips. 3) VIP may act as a neurotransmitter-neuromodulator of non-adrenergic inhibitory nerve, while SP and Neurotensin may act as that of non-cholinergic excitatory nerve with some direct effect on the intestinal muscle.
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PMID:[Role of the peptidergic nerves in Hirschsprung's disease and hypoganglionosis]. 248 12

Intrathecal administration of VIP to the thoracic spinal cord in the urethane anaesthetized rat provoked a dose-dependent increase in heart rate without any change in arterial pressure. The cardioacceleration observed following administration of 6.5 nmol of VIP at the T9 level (n = 8) occurred within 1-2 min of administration, with a peak effect of 70-85 bpm, 10-30 min after administration. The magnitude of the maximum change when this dose was given at the T2 level (n = 8) was approximately 100 beats per min, 7-8 min after administration. However, the differences between T2 and T9 administration were not statistically significant. Intravenous administration of 6.5 nmol of VIP (n = 6) mimicked the cardioacceleratory effect of intrathecal administration, and also decreased systolic and diastolic arterial pressure by 9-13 mmHg 6-13 min after administration. The cardioacceleration observed following intrathecal administration at T9 was not blocked by prior systemic administration of the autonomic ganglion blocker hexamethonium (5 mg/kg) or by bilateral vagotomy. Nor was the effect blocked by prior intrathecal administration of the local anaesthetic lidocaine (250 micrograms), although lidocaine did block the tachycardia and hypertension resulting from intrathecal administration of substance P. Considered collectively, the findings that the cardioacceleration observed following intrathecal VIP injection is mimicked by i.v. administration, is not reversed by blockade of nicotinic transmission of autonomic ganglia or by bilateral vagotomy, and is not blocked by lidocaine suggest that VIP's tachycardic effect does not result from a direct action on spinal mechanisms mediating autonomic control of the cardiovascular system, but occurs via diffusion to a site of action outside the central nervous system.
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PMID:Cardioacceleration provoked by intrathecal administration of vasoactive intestinal peptide (VIP): mediation by a non-central nervous system mechanism. 248 50

By immunocytochemistry a number of the gut/brain peptides have been demonstrated in nerve fibers of the mammalian urogenital tract. These peptides are localized to large vesicles in nerve terminals of afferent fibers or efferent nerves innervating blood vessels, non-vascular smooth muscle, lining epithelium and glands. There is evidence that some neuropeptides (VIP, NPY) participate in the local non-cholinergic, non-adrenergic nervous control of smooth muscle activity and blood flow, while other peptides (substance P, CGRP) seem to be sensory transmitters. It is likely that impaired function of the peptidergic nerves is involved in sexual dysfunction such as male impotence.
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PMID:Regulatory peptides in the mammalian urogenital system. 252 94

Neuropeptide Y (NPY)-immunoreactive (IR) nerve fibres were found around both arteries and veins and in smooth muscle trabeculae of the cat spleen with the highest density on the arterial side. Considerably more tyrosine hydroxylase (TH)- and dopamine-beta-hydroxylase (DBH)-positive than NPY-IR nerves were seen in the trabeculae and splenic capsule. The NPY-IR nerves in the spleen most likely originated in the coeliac ganglion, since (1) splanchnic nerve sectioning did not change the splenic NPY-IR nerves, (2) most neurones in the coeliac ganglion were NPY-IR, as well as DBH- and TH-positive, and (3) NPY-IR was transported axonally from the coeliac ganglion towards the spleen via the splenic nerve. Local NPY infusion in the isolated, blood-perfused cat spleen caused a marked increase in splenic vascular resistance and a small volume reduction. NA caused a comparatively larger reduction in splenic volume than NPY in addition to vasoconstriction. VIP-IR cell bodies in the coeliac ganglion were NPY- and TH-negative. VIP-IR nerves were seen both around the splenic artery and vein as well as around arterioles and within venous trabeculae of the spleen. VIP infusion caused reduction of splenic perfusion pressure (i.e. vasodilation) as well as an increase in splenic volume. Substance P-IR nerves, most likely of splanchnic afferent origin, were present in the coeliac ganglion around the splenic artery and arterioles of the spleen. Infusion of substance P induced marked reduction in perfusion pressure and a reduction in splenic volume. Enkephalin-immunoreactive nerves of splanchnic origin surrounded some TH- and NPY-positive, coeliac ganglion cells. It is concluded that several vasoactive peptides are located in splenic nerves. NPY is present in noradrenergic neurones and causes mainly increased vascular resistance. VIP occurs in non-adrenergic neurones of sympathetic origin and induces vasodilation and relaxation of the capsule. Finally, substance P is present in peripheral branches of spinal afferent nerves and causes vasodilation and capsule contraction. Stimulation of the splenic nerves may thus release several vasoactive substances in addition to noradrenaline, exerting a variety of actions.
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PMID:Neuropeptide Y-, substance P- and VIP-immunoreactive nerves in cat spleen in relation to autonomic vascular and volume control. 257 17

The present study was done both in vivo by cannulating pancreatic duct of rats and in vitro using pancreatic slices and dissociated acini to determine the mode of action of endogenous opiate peptides on pancreatic acinar cell. Pancreatic slices were incubated with beta-endorphin or (Met)5-enkephalin alone and in combination with CCK8. Dissociated acini were incubated with naloxone, substance P, VIP, (Met)5- and (Leu)5-enkephalin and alpha-, beta-, and gamma-endorphin alone or in combination with CCK8. In vivo, both beta-endorphin and (Met)5-enkephalin did not alter basal secretion but inhibited CCK8-stimulated amylase secretion. This effect was not reversed by administration of naloxone. In the slices, neither beta-endorphin nor (Met)5-enkephalin altered basal or CCK8-stimulated secretion. In the dissociated acini, substance P and VIP significantly increased amylase secretion, whereas naloxone, enkephalins, and endorphins failed to alter amylase secretion. CCK8 increased amylase secretion greater than sixfold. In combination with enkephalins and endorphins, there was neither inhibition nor potentiation of CCK8 effect. These data indicate that the effect of opiate peptides on pancreatic acinar cells in the rat are nonspecific and appear not to be mediated by opiate receptors.
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PMID:Effect of endorphins on amylase secretion from rat pancreas in vivo and in vitro. 257 22

The innervation of the ductuli efferentes and seven zones of the guinea-pig epididymis was investigated using immunohistochemical, histochemical and electron-microscopical techniques. Nerve fibers were localized by use of antibodies against substance P (SP-IR), vasoactive intestinal polypeptide (VIP-IR) and dopamine-beta-hydroxylase (DBH-IR). In the ductuli efferentes and all zones of the epididymal duct, SP-IR is consistently observed in the interstitial tissue and perivascular areas. Histochemistry reveals a significant amount of acetylcholinesterase-containing fibers in the interstitial, perivascular and periductal smooth muscles of the ductuli efferentes and zones V, VI and VII. In contrast to the homogeneous distribution of SP-IR within all zones of the epididymis, VIP-IR is seen only in zones VI and VII. Within these zones, VIP-IR is detected in large amounts in the subepithelial and muscular layers as is a sparse number of SP-IR varicosities. DBH-IR is also seen throughout all zones in the interstitial and perivascular regions with a tendency to increase in zones VI and VII. Transmission electron microscopy (TEM) reveals evidence of a cholinergic (agranular vesicles, AGV), adrenergic (small granular vesicles, SGV) and peptidergic (large granular vesicles, LGV) innervation throughout the interstitial connective tissue of the ductuli efferentes and all epididymal zones. Furthermore AGV are localized in the subepithelial layer, and also co-stored with LGV in the muscular layer of zones VI and VII. No nerve profiles were encountered within the epithelium. A correlation of immunohistochemical findings to TEM counterparts as well as their possible functional role are discussed.
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PMID:Studies of the guinea-pig epididymis. III. Innervation of epididymal segments. 257 39

Sections of rat superior and recurrent laryngeal nerves (SLN and RLN) with enclosed paraganglia and ganglionic cells were incubated with antisera against five different neuropeptides. Vasoactive intestinal polypeptide-like immunoreactivity (VIP-LI) and neuropeptide Y (NPY)-LI was detected in a large number of varicose nerve fibres in the paraganglia. A few varicosities of the paraganglia showed substance P (SP)-LI or calcitonin gene-related peptide (CGRP)-LI, whereas there were no signs of enkephalin (ENK)-LI in these varicosities. The paraganglionic cells never exhibited immunoreactivity for any of the peptides tested, whereas some of the associated ganglionic cells showed NPY-LI, VIP-LI or ENK-LI. The study shows that the paraganglia of the SLN and RLN receive a significant peptidergic innervation and suggests that the peptide-containing nerve fibres in these structures originate from cells other than the paraganglionic cells. The findings imply that in further studies defining the function of laryngeal nerve paraganglia in larynx physiology, the role of neuropeptides should be examined.
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PMID:Networks of peptide-containing nerve fibres in laryngeal nerve paraganglia. An immunohistochemical study. 264 51

Sympathetic and parasympathetic influences on the airway resistance under physiological and pathophysiological conditions have long been known. In recent years, this classical view had to be extended due to mounting evidence of neurocrine and paracrine peptide mediators. The term non-adrenergic non-cholinergic (NANC) nervous system was coined. Besides other effects the non-adrenergic mediators (e.g. VIP and PHI/PHM) give rise to bronchodilation, while the non-cholinergic modulators (SP, neurokinin A, and CGRP) induce bronchospasm. The axon-reflex theory postulates liberation of non-cholinergic peptide substances by afferent C-fibers exposed by bronchial epithelial cell damage as one important cause of bronchial obstruction. In addition to biogenic amines, such peptides as bombesin, leu-encephalin, beta-endorphin, calcitonin, doctrine cells of the bronchial epithelium. Our knowledge of the biological relevance of these mediators is at present very sketchy. Platelet activating factor (PAF) is released by alveolar macrophages, granulocytes, blood vessel endothelium, and platelets. The inhalation of PAF induces bronchospasm in healthy subjects and asthmatics and also prolonged bronchial hyperreactivity. The many factors influencing bronchial reactivity need to be classified by further investigations of the mode of interaction and interdependence of known and new mediators.
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PMID:[The significance of endocrine, neurocrine and paracrine mediators in the regulation of bronchial reactivity]. 268 1

When cytoplasmic extracts of the myenteric plexus of guinea pig ileum are submitted to centrifugal density gradient separation in a zonal rotor, conditions which separate storage particles containing substance P, somatostatin and VIP from each other, PHI copurifies with VIP. The two immunoreactivities cannot be separated by particle exclusion chromatography, which depends on size rather than density. It is concluded that the posttranslational cleavage of the propeptide or precursor to PHI and VIP occurs after packaging into these storage particles.
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PMID:A peptide with N-terminal histidine and C-terminal isoleucine amide (PHI) and vasoactive intestinal peptide (VIP) are copackaged in myenteric neurones of the guinea pig ileum. 278 Apr 16

The intrinsic innervation of the colon is provided by enteric plexi, of which some axons form the neuro-enterocytic junction and others are interconnecting. The extrinsic innervation depends on the autonomous para- and ortho-sympathetic nervous system and is modulated and administered by the central nervous system. Neurotransmission occurs at the level of the mucosa through acetyl-cholin and VIP, among others. At the level of the sub-mucous ganglia, serotonin and enkephalins would play a role. Acetyl-cholin and, probably VIP are neurotransmitters of the para-sympathetic system; nor-adrenalin, dopamine, NPY and histamine are neurotransmitters of the orthosympathetic system. Prevertebral ganglia, relay of the orthosympathetic system, also have an activity per se, influenced by VIP and substance P. Many neuropeptides of the central nervous system have a presumed and not yet identified role of administration. Various diseases in which the enteric plexi are destroyed, are described.
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PMID:[Colonic neuroendocrinology]. 282 70


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