Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: UNIPROT:P20366 (substance P)
 21,176 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

The development of the putative neurotransmitter substance P (SP) in rat dorsal root ganglion (DRG) was defined in vivo. The sixth cervical DRG of newborn rats contained 70 pg of SP, and the ganglionic content increased 5.5-fold during the first 5 weeks of life. Forelimb amputation partially prevented the normal developmental increase of SP in the sixth cervical DRG destined to innervate that limb. Conversely, treatment with nerve growth factor (NGF) increased both ganglionic SP and total ganglion protein. Moreover, NGF administration prevented the failure of SP development that followed amputation, suggesting that NGF may mediate the limb-DRG interaction. However, treatment with antiserum to NGF failed to significantly inhibit development of ganglion SP. Consequently, neonatal ganglia may remain responsive to NGF, without requiring the protein for survival. SP appears to be an excellent index of the maturation of neurons in dorsal root ganglia.
 ...
PMID:Nerve growth factor stimulates the development of substance P in sensory ganglia. 615 99

The protein nerve growth factor (NGF) is known to be essential for the maturation and maintenance of adrenergic neurones and for the development of sensory neurones during critical stages of embryonic life. The investigation of the physiological importance of NGF for the development of sensory neurones has been hampered so far by the lack of biochemical marker substances for these neurones. The demonstration that the undecapeptide substance P(SP) is present in sensory neurones suggests that it might be such a marker. SP is synthesized in dorsal root ganglia (DRG) and transported to the terminals of C-fibres located in the dorsal horn of the spinal cord and in the skin. Its release can be demonstrated from the central and peripheral endings of sensory nerve fibres which seem to have an important role in pain perception. We have investigated the effects of NGF and of purified anti-NGF antibodies on the content of SP in rat DRG and in their respective target organs, namely the spinal cord and the skin. The effects on sympathetic ganglia were included in order to control the effectiveness of both NGF and its antibody. We report here that NGF leads to an increase in SP in spinal ganglia, as previously shown by Kessler and Black. However, in contrast to these authors, we describe that the administration of anti-NGF antibodies produces a marked reduction of the SP content in sensory neurones and in their respective nerve terminals.
 ...
PMID:Requirement of nerve growth factor for development of substance P-containing sensory neurones. 615 33

Development of the putative neurotransmitter, substance P (SP), in the embryonic rat dorsal root ganglion (DRG) and spinal cord was defined in vivo. SP was not detectable by radioimmunoassay before day 17 of gestation (E17). On E17, cervical sensory ganglia contained 4 pg SP/ganglion, rising to 49 pg/ganglion at birth. The dorsal cervical spinal cord contained 0.75 ng SP/mg protein on E17, rising to 6 ng SP/mg protein on postnatal day 3. The ventral spinal cord contained approximately 20% of the SP content in the dorsal cord at each gestational age. Intrauterine forelimb amputation partially prevented the normal development increase of SP in sensory ganglia destined to innervate that limb, suggesting that target structures regulate the development of peptidergic neruons. Conversely, treatment with nerve growth factor (NGF) stimulated development of SP in the DRG. Moreover, NGF treatment increased SP in the dorsal spinal cord, suggesting that NGF can modulate development within the CNS, as well as peripheral structures. It is likely that the CNS effect reflects NGF peptidergic neruons. Conversely, treatment with nerve growth factor (NGF) stimulated development of SP in the DRG. Moreover, NGF treatment increased SP in the dorsal spinal cord, suggesting that NGF can modulate development within the CNS, as well as peripheral structures. It is likely that the CNS effect reflects NGF peptidergic neruons. Conversely, treatment with nerve growth factor (NGF) stimulated development of SP in the DRG. Moreover, NGF treatment increased SP in the dorsal spinal cord, suggesting that NGF can modulate development within the CNS, as well as peripheral structures. It is likely that the CNS effect reflects NGF action on peripheral ganglia, but a direct effect on the spinal cord has not been excluded. However, treatment with antiserum to NGF failed to significantly inhibit development of ganglion SP. The system of SP-containing neurons in the DRG may provide a convenient model for defining events regulating peptidergic maturation.
 ...
PMID:Nerve growth factor stimulates development of substance P in the embryonic spinal cord. 616 31

Development of the two putative peptide neurotransmitters, substance P (SP) and somatostatin (SS), were compared in rat dorsal root ganglion (DRG) and spinal cord in vivo. The content of SS in the sixth cervical DRG increased 5-fold during the first 5 weeks of life, rising from 24 pg per ganglion at birth. SP content increased 4.5-fold during the first 5 weeks, from 56 pg per ganglion at birth. The developmental profiles for these two peptides were virtually parallel, suggesting that their respective neuronal populations developed in synchrony. Treatment with nerve growth factor (NGF) significantly increased the content of both SP and SS in the DRG and dorsal spinal cord. Conversely, treatment with capsaicin significantly decreased both SP and SS in the DRG and dorsal spinal cord. Consequently, experiments involving NGF or capsaicin treatment of sensory neurons must be interpreted with extreme care, because specificity is not limited to a single peptide phenotype. Although the mechanisms of action of NGF and capsaicin on SP and SS have not been defined, the similarity of the responses of the two peptides suggests that their development may be regulated by similar processes.
 ...
PMID:Similarities in development of substance P and somatostatin in peripheral sensory neurons: effects of capsaicin and nerve growth factor. 617 69

Nerve growth factor is retrogradely transported in sympathetic and sensory neurons throughout life. Although this transport is known to be biologically significant in sympathetic neurons, such a function was not yet known in sensory ganglia. By using the neuropeptide substance P as a biochemical marker, we show that sensory ganglia from newborn and adult rats respond in nerve growth factor and that its retrograde axonal transport is biologically relevant, as indicated by an increase in substance P and in general protein content.
 ...
PMID:Biological importance of the retrograde axonal transport of nerve growth factor in sensory neurons. 617 Sep 88

Rats exposed to maternal antibodies against nerve growth factor (NGF) in utero and in milk have been used to investigate the developmental dependency of various substance P-containing neurons on NGF. Substance P and other peptides were measured by radioimmunoassays. The substance P content of sensory ganglia, spinal cord, and skin was depleted 40 to 60% in anti-NGF-treated rats. These results demonstrate the NGF dependence of substance P-containing neurons in sensory ganglia. Opiate binding in the spinal cord was not changed despite the large depletion in substance P: the Bmax and KD were the same in control and treated animals. The results suggest that opiate receptors may not be located presynaptically on substance P-containing primary afferents. Among the peripheral tissues which were assayed (ileum, submaxillary gland, retina, and adrenal), substance P decreased only in the adrenal, suggesting innervation by a NGF-dependent substance P-containing neuron. No changes were detected in the substance P content of nine different brain regions, in agreement with previous observations on the lack of effect of NGF on central neurons.
 ...
PMID:Use of an experimental autoimmune model to define nerve growth factor dependency of peripheral and central substance P-containing neurons in the rat. 617 32

Administration of anti-nerve growth factor (NGF)-antibodies to newborn rats produces a marked but reversible reduction of the substance P content in dorsal root ganglia. This is in contrast to the effect of anti-NGF-antibodies on sympathetic ganglia, where they cause a destruction of the adrenergic neurons as is evident in the irreversible reduction of tyrosine hydroxylase activity.
 ...
PMID:Effects of antibodies against nerve growth factor on the postnatal development of substance P-containing sensory neurons. 617 29

Capsaicin depleted substance P from guinea pig dorsal root ganglia and inhibited the retrograde axoplasmic transport of nerve growth factor (NGF). Doses of capsaicin which depleted substance P also inhibited the retrograde axoplasmic transport of NGF. Inhibition of the retrograde transport of NGF by capsaicin preceded substance P depletion. Supplementation of guinea pigs with mouse NGF completely prevented capsaicin-induced substance P depletion. It is concluded that capsaicin depletes substance P from primary afferent neurons of the adult guinea pig by altering the availability of NGF. The data support a role for NGF in the normal maintenance of neuropeptide levels in some sensory neurons in the adult animal.
 ...
PMID:Regulation of substance P by nerve growth factor: disruption by capsaicin. 618 49

An experimental autoimmune model was used to study effects of nerve growth factor deprivation on the peptide content of sensory neurones and on pain sensitivity. Rats exposed during development to anti-nerve growth factor antibodies showed an increased threshold towards nociceptive stimuli. These animals also showed a significant decrease in substance P levels in their dorsal root ganglia and spinal cord. These results suggest that substance P-containing primary sensory neurones are involved in certain types of nociception.
 ...
PMID:Correlation between substance P content of primary sensory neurones and pain sensitivity in rats exposed to antibodies to nerve growth factor. 618 53

The protein nerve growth factor (NGF) is a naturally occurring trophic substance for sympathetic neurones and for at least those primary sensory neurones containing substance P (refs 4-6). Thus retrogradely transported NGF increased substance P and protein content in corresponding dorsal root ganglia. Moreover, anti-NGF antibodies administered to newborn rats decreased substance P and somatostatin levels in dorsal root ganglia and dorsal spinal cord, suggesting an important role for NGF in the postnatal development of peptidergic sensory neurones. These neurones appear to be selectively affected by the neurotoxin capsaicin (8-methyl-N-vanillyl-6-nonenamide). Treatment of newborn rats with capsaicin led to degeneration of primary sensory neurones containing substance P, somatostatin, vasoactive intestinal polypeptide and cholecystokinin. The mechanism by which capsaicin evokes its neurotoxic effect is unknown. We report here that in newborn rats concomitant administration of NGF partially antagonized the deleterious effect of capsaicin on substance P-containing neurones in dorsal root ganglia as assessed by morphological and biochemical criteria. We conclude that capsaicin destroys the perikarya of primary sensory peptidergic neurones by interfering with the action of NGF, probably by blocking its retrograde axonal transport.
 ...
PMID:Nerve growth factor antagonizes the neurotoxic action of capsaicin on primary sensory neurones. 618 53


<< Previous 1 2 3 4 5 6 7 8 9 10 Next >>