Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UNIPROT:P20366 (substance P)
 21,176 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

The effects of vasoactive intestinal polypeptide (VIP) and substance P on isolated human intramyometrial arteries and fetal stem villous arteries obtained from term pregnant women were compared. Ring preparations of small intramyometrial arteries and fetal stem villous arteries obtained at caesarean section were mounted in organ baths, and isometric tension was recorded. None of the peptides affected resting tension. In intramyometrial arteries precontracted by vasopressin (2.8 x 10(-9) M) both substance P (10(-12) to 10(-8) M) and VIP (10(-8) to 10(-6) M) caused relaxation. In fetal stem villous arteries precontracted by prostaglandin F2 alpha (10(-5) M) cumulative addition of substance P (10(-11) to 10(-6) M) did not produce significant changes in tension as compared with controls, while addition of single doses produced moderate relaxation. VIP (10(-8) to 10(-6) M) induced relaxation with similar effects for the addition of cumulative and single doses. The responses to VIP and substance P remained unaffected after pretreatment by atropine (10(-6) M), propranolol (10(-6) M), and indomethacin (10(-6) M). The results support a role for VIP and substance P in the regulation of uteroplacental blood flow in term pregnancy.
Placenta
PMID:Effects of vasoactive intestinal polypeptide and substance P on human intramyometrial arteries and stem villous arteries in term pregnancy. 246 22

The occurrence of methionine enkephalin (379 pg/g tissue), beta-endorphin (448 pg/g tissue) and Substance P (2.4 pg/g tissue) in human placental villus were demonstrated by sensitive and specific radioimmunoassays. Conditions for the bioassay of placental extracts for enkephalin-like activities using the rat vas deferens were described. Substance P did not interfere in this bioassay. Comparison of the enkephalin-like activities determined by bioassay and the content of beta-endorphin and methionine enkephalin determined by radioimmunoassays indicated that placental villus extracts contain other unidentified potent opioid-like peptides or substances. It is suggested that methionine enkephalin and/or beta-endorphin and Substance P regulate release of acetylcholine or hormones from placental villus. Alternatively, these peptides may regulate sensory transmission (pain impulses) locally from the distended uterus during pregnancy or from the vaginal tract during childbirth.
Placenta Suppl 1981
PMID:Peptides from human placenta: methionine enkephalin and substance P. 619 22

Endothelium derived relaxing factor (EDRF), now widely believed to be nitric oxide (NO), may play an important part in the control of fetoplacental vascular tone. To further explore this role we have determined the relaxation responses to exogenous NO and examined the temporal relationship between intracellular concentrations of cyclic GMP and vascular tone in isolated ring segments of human chorionic plate arteries. We have also determined the dose relations for the contractile agonists serotonin and the thromboxane analog U46619. Lastly, we have explored the relaxation responses to a wide range of agents known to elicit EDRF release in other vascular beds. Chorionic plate arteries relaxed significantly to exogenous NO with concomitant increases in cyclic guanosine monophosphate over basal values. ED50s for serotonin and U46619 were 1.48 x 10(-6) M and 3.39 x 10(-8) M respectively. The ED50 for NO derived from S-nitroso-N-acetyl-penicillamine was 1.28 x 10(-6) M. Endothelium-intact segments of chorionic plate arteries pre-contracted with either serotonin or U46619 failed to relax significantly to acetylcholine, adenosine diphosphate, A23187, bradykinin, and histamine and only minimally to substance P. We suggest that EDRF is likely to be important in the control of placental vascular tone, but that it is not possible to demonstrate its action in an unperfused experimental system.
Placenta 1994 Jun
PMID:Endothelium-derived relaxing factor and cyclic GMP-dependent vasorelaxation in human chorionic plate arteries. 752 60

Human umbilical vessels are devoid of nerves and therefore endothelial cells may play an important role in the control of fetoplacental blood flow. In this study we examined the pharmacological effects of various substances, known to produce endothelial-mediated vasodilation in many blood vessels, on the human umbilical artery and vein from legal terminations [mean gestational age, 15 (8-17) weeks; n = 12] and normal term vaginal deliveries [mean gestational age, 39 (38-41) weeks; n = 12]. Acetylcholine, adenosine 5'-triphosphate, the calcium ionophore A23187 and substance P had no effect on raised vascular tone, whereas sodium nitroprusside relaxed 5-hydroxytryptamine (5-HT) preconstricted, umbilical artery and vein from both early and late pregnancy. L-NG-Nitroarginine methyl ester (L-NAME) had no effect on basal tone or on high tone, after it was raised by 5-HT. Localization of nitric oxide synthase [NOS, type I (neuronal)] was examined in the same umbilical vessels using electron immunocytochemistry. No NOS-immunoreactive endothelial cells were observed in the umbilical vessels taken during early pregnancy. However, the percentage of NOS-immunoreactive endothelial cells in umbilical artery and vein from late pregnancy was 3 and 10 per cent, respectively. These results suggest that nitric oxide contributes little, if any, to the local control of umbilical blood flow throughout pregnancy, despite the presence of NOS-immunoreactivity in a subpopulation of endothelial cells in late pregnancy.
Placenta 1995 Apr
PMID:Nitric oxide and human umbilical vessels: pharmacological and immunohistochemical studies. 763 9

Neuropeptides play an important role in the regional regulation of blood flow and hormone secretion. Few studies report the presence of peptides in the human placenta. Our experiment evaluates neuropeptides in the human placenta using immunocytochemical techniques. Representative tissue sections from full-term placentae were fixed immediately after delivery and processed into paraffin sections or frozen. They were treated with multiple immunofluorescence, streptavidin-biotin-peroxidase complex and immunogold-silver staining techniques in combination with well-established monoclonal and polyclonal antibodies, using appropriate absorption controls to ensure the validity of the staining. Vasoactive intestinal polypeptide (VIP), calcitonin gene-related peptide (CGRP), neuropeptide tyrosine (NPY), galanin, somatostatin, met-enkephaline, helodermin and substance P-like immunoreactivities were demonstrated within decidual cells. Endothelin-1 was found in both trophoblasts and endothelial cells. Peptide immunoreactivities in the human placenta especially at the decidual interface between mother and fetus supports a role for the diffuse neuroendocrine system (DNES) in the regulation of placental blood flow critical for fetal growth and development.
Placenta 1996 Sep
PMID:Localization and distribution of vasoactive neuropeptides in the human placenta. 889 70

A role for Substance P (SP) in human placenta is not known, although is possible that regulates placental physiology through the Neurokinin (NK)-1 receptor. Ten human normal placenta tissues were studied by immunohistochemistry to demonstrate the localization of NK-1 receptor and SP. An immunostaining pattern for NK-1 receptor and SP was observed in the endothelium and myocytes of fetal blood vessels, decidua and trophoblast. The SP is located in both the cytoplasm and the nucleus whereas NK-1 receptor in cytoplasmic. These findings reported here for the fist time, suggest a role for the SP and NK-1 receptor in the placental physiology.
Placenta 2010 Jul
PMID:Immunolocalization of NK-1 receptor and Substance P in human normal placenta. 2043 Apr 40