Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: UNIPROT:P20226 (TATA-binding protein)
 1,297 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Hormone activation of MMTV transcription results in the establishment of a tightly bound transcription factor complex at the promoter (Cordingley et al., Cell 48, 261-270, 1987). We have characterized two fractionable binding activities which participate in this complex. One factor, previously identified as the mouse homologue of NF-1 (or CTF), protects sequences -82 to -56 from exonuclease III digestion in vitro. Sequences protected by a second factor (-42 to -4) span the TATA box of the promoter, suggesting that the binding activity in this fraction is equivalent to the HeLa cell transcription factor TFIID (Sawadogo and Roeder, Cell 43, 165-175, 1986). The downstream boundary of exonuclease protection by the putative TATA-binding factor is -4; DNase1 footprinting of this fraction, however, showed additional protection of discrete sites downstream of the cap site. The apparent concentration and promoter-specific binding activity of both factors is unaffected by hormone treatment of the cells.
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PMID:Binding of multiple factors to the MMTV promoter in crude and fractionated nuclear extracts. 282 33

Our previous studies isolated and characterized eight splicing variants containing exon 11 as the first coding exon. The location of exon 11 about 10 kb upstream from exon 1 dual promoters implied another promoter to drive expression of the exon 11 variants. I now identify the second promoter in the 5'-flanking region of exon 11. One major transcriptional start point was determined. Sequence analysis indicated that the 5'-flanking region of the exon 11 contained a putative TATA box, several CAAT boxes and a number of cis-acting elements. Functional analysis suggested that exon 11 promoter activity was most evident in neuronal-like cells. A basal core region containing the TATA box, a negative region and a positive region were identified. Electrophoretic mobility shift assays with the nuclear extracts from NIE-115 cells revealed several protein complexes that likely contained TATA box-associated factors, NF-1-like and cMyc-Max-like proteins, respectively. It also showed that a TATA-binding protein specifically bound to the TATA box fragment. Mutation analysis suggested that the TATA box in the basal core region played a fundamental role in the exon 11 promoter, whereas the NF-1 site acted as a positive element.
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PMID:Identification and characterization of a novel promoter of the mouse mu opioid receptor gene (Oprm) that generates eight splice variants. 1224 16