Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: UNIPROT:P20226 (
TATA-binding protein
)
1,297
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
The heterodimeric transcription factor E2F1-
DP1
plays crucial roles in coordinating gene expression during G
1
/S cell cycle progression. For transcriptional activation, the transactivation domain (TAD) of E2F1 is known to interact with the
TATA-binding protein
of TFIID and the p62 subunit of TFIIH. It is generally believed that
DP1
facilitates E2F1 binding to target DNA and does not possess a TAD. Here, we show that an acidic region of
DP1
, whose function has remained elusive, binds to the plekstrin homology (PH) domain of p62 with higher affinity than that of E2F1 and contributes to transcriptional activation. The structure of the complex revealed that
DP1
forms a twisted U-shaped, string-like conformation and binds to the surface of the PH domain by anchoring Phe403 into a pocket in the PH domain. The transcriptional activity of E2F1-
DP1
was reduced when Phe403 of
DP1
was mutated. These findings indicate that the acidic region of
DP1
acts as a TAD by contacting TFIIH.
...
PMID:The Interaction Mode of the Acidic Region of the Cell Cycle Transcription Factor DP1 with TFIIH. 2782 26
