Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
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Target Concepts:
Gene/Protein
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Query: UNIPROT:P20020 (
adenosine triphosphatase
)
3,299
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
The vacuolar H
+
-
adenosine triphosphatase
(
ATPase
) subunit V0C (
ATP6V0C
), a proton-conducting, pore-forming subunit of vacuolar ATPase, maintains pH homeostasis and induces organelle acidification. The intracellular and extracellular pH of cancer cells affects their growth; however, the role of
ATP6V0C
in highly invasive esophageal cancer cells (ECCs) remains unclear. In this study, we examined the role of
ATP6V0C
in glucose metabolism in ECCs. The
ATP6V0C
depletion attenuated ECC proliferation, invasion, and suppressed glucose metabolism, as indicated by reduced glucose uptake and decreased lactate and adenosine triphosphate (ATP) production in cells. Consistent with this, expression of glycolytic enzyme and the extracellular acidification rate (ECAR) were also decreased by
ATP6V0C
knockdown. Mechanistically,
ATP6V0C
interacted with pyruvate kinase isoform M2 (PKM2), a key regulator of glycolysis in ECCs. The
ATP6V0C
depletion reduced PKM2 phosphorylation at tyrosine residue 105 (Tyr
105
), leading to inhibition of nuclear translocation of PKM2. In addition,
ATP6V0C
was recruited at hypoxia response element (HRE) sites in the lactate dehydrogenase A (
LDHA
) gene for glycolysis. Thus, our data suggest that
ATP6V0C
enhances aerobic glycolysis and motility in ECCs.
...
PMID:Vacuolar H
+
-ATPase Subunit V0C Regulates Aerobic Glycolysis of Esophageal Cancer Cells via PKM2 Signaling. 3155 33
