Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: UNIPROT:P20020 (adenosine triphosphatase)
 3,299 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Embryonal rhabdomyosarcomas from the nasopharynx of two children were examined by histochemical methods commonly applied to muscle biopsies. These stains included nicotinamide adenine dinucleotide-tetrazolium reductase (NADH-TR), succinate dehydrogenase (SDH), PAS, PAS-diastase, myophosphorylase, calcium-mediated adenosine triphosphatase (ATPase) preincubated at high and low pH, and oil red O. Myofibrils were easily identified with ATPase and blood vessel walls were also stained. NADH-TR clearly showed longitudinal and cross-striations that were not seen with H&E or PTAH stains. The modified Gomori trichrome stain additionally contributed to the recognition of myofibrils. Some techniques of muscle histochemistry applied to fresh frozen sections of tumor tissue may provide evidence of muscular differentiation in otherwise poorly differentiated sarcomas for a more accurate diagnosis of rhabdomyosarcoma.
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PMID:Diagnostic value of histochemistry in embryonal rhabdomyosarcoma. 9 52

Six rhabdomyosarcomas were assessed by means of a battery of enzyme histochemical methods. The reactions were compared with those of a small number of other tumours belonging to the small-cell tumour category. Four of the rhabdomyosarcomas were positive for myophosphorylase and acetylcholinesterase. Myoblasts were strongly reactive for adenosine triphosphatase at alkaline pH and after acid pre-incubation, whereas the small undifferentiated neoplastic cell of the four alveolar rhabdomyosarcomas showed also discernible cytoplasmic reaction, but only after acid pre-incubation. Other tumour categories revealed positive staining for adenosine triphosphatase with acid pre-incubation but the degree of reaction was minimal by comparison. Other enzyme reactions were variable and, generally, did not distinguish between different tumour categories. It is concluded that enzyme histochemistry has a potential role in the diagnostic evaluation of the small cell tumour and should be included in the growing list of special techniques that may assist the pathologist confronted with this problem.
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PMID:An evaluation of enzyme histochemistry in the diagnosis of childhood rhabdomyosarcoma. 315 41