Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: UNIPROT:P20020 (
adenosine triphosphatase
)
3,299
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Methanethiol
(
CH3SH
) has been implicated in the pathogenesis of hepatic coma. Studies are presented to identify the possible biochemical basis of anesthesia-like effects of
methanethiol
and those features which distinguish such effects from common anesthetics and may represent the basis of its toxicity.
CH3SH
was found to stabilize erythrocyte membranes against hypotonic hemolysis at relatively low concentrations. At 37 degrees C the AH25 value for human erythrocyte antihemolysis was observed at a concentration of 0.34 mumol of
CH3SH
bound per mg of erythrocyte protein. Similar results were obtained with rat erythrocytes. This property of
CH3SH
is in common with other anesthetic agents. Anesthetic agents also inhibit the membrane-associated Na+,K+-
adenosine triphosphatase
(
ATPase
); however, for effective and nontoxic agents of this type the inhibition of
ATPase
activity is elicited at concentrations which are at least an order of magnitude higher than those which influence the membrane stability characterized by the antihemolysis effect (P. Seeman, Pharmacol. Rev. 24: 583-655, 1972).
CH3SH
was also found to inhibit the membrane Na+,K+-
ATPase
activity. The I25 value for the inhibition of human erythrocyte
ATPase
activity was obtained at
CH3SH
concentration of 0.12 mM which corresponded to 0.3 mumol of
CH3SH
bound per mg of erythrocyte membrane protein. Rat erythrocyte membrane
ATPase
was somewhat more sensitive to
CH3SH
. In all cases the binding of
CH3SH
to erythrocytes occurred primarily on the membrane. These results indicate that no differential exists with respect to the dose-response of these two activities associated with human erythrocyte membrane.(ABSTRACT TRUNCATED AT 250 WORDS)
...
PMID:Effects of methanethiol on erythrocyte membrane stabilization and on Na+,K+-adenosine triphosphatase: relevance to hepatic coma. 631 65
