Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UNIPROT:P20020 (adenosine triphosphatase)
 3,299 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

p-Aminohippurate (PAH) uptake by teased flounder renal tubules was concentrative, Na-dependent, dependent on aerobic metabolism and inhibited by competitor organic anions and ouabain. Dose-response data for ouabain inhibition of PAH transport and tubule Na,K-adenosine triphosphatase (ATPase) activity were identical. In ouabain-treated tubules, reductions in PAH uptake correlated with alterations in total tissue Na and K even though both were not affected during the first 5 to 10 min of ouabain exposure. No such delay was found with HgCl2 and, as with ouabain, reductions in transport correlated well with alterations in tissue Na and K. Tissue respiration data indicated that low concentrations of HgCl2 rapidly affected the Na,K-ATPase. With higher HgCl2 concentrations, intracellular metabolic sites appeared to be affected. Transport studies with ouabain-poisoned tubules and plasma membrane vesicles indicated that the energetically uncoupled PAH carrier(s) were affected by HgCl2, but carrier sensitivities to Hg were lower than for the Na,K-ATPase. The data indicate that HgCl2 inhibits PAH transport primarily by reducing ion gradients that drive PAH transport. Both inhibition of Na,K-ATPase and increases in membrane permeability to Na and K appear to be involved.
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PMID:Heavy metal inhibition of p-aminohippurate transport in flounder renal tissue: sites of HgCl2 action. 627 Mar 7

Sodium vanadate is a potent inhibitor of Na-K-adenosine triphosphatase. p-Aminohippurate (PAH) and tetraethylammonium accumulation in rat renal cortical slices was inhibited by vanadate in a dose-dependent manner at medium vanadate concentrations from 10(-6) to 10(-3) M. Inhibition was reversible at vanadate concentrations less than 1.5 x 10(-5) M. The slice content of vanadium (7.5-325 micrometer V/g wt. of tissue) was linearly related to medium vanadate concentrations ranging from 10(-5) to 10(-3) M. The ability of slices to generate glucose and ammonia was not impaired by medium vanadate concentrations up to 5 x 10(-4) M, a concentration that maximally inhibited organic ion accumulation. Increasing medium K+ concentrations potentiated vanadate inhibition of PAH accumulation which correlated with inhibition of sodium pump activity, as determined by 42K+ uptake. Intraperitoneal administration of vanadate (1 or 5 mg V/kg) to rats produced a profound diuresis and natriuresis during the 1st hr. Inhibition of PAH accumulation of renal slices from these rats was related to tissue vanadium concentrations. These data suggest that vanadate exerts its action on proximal tubule transport of PAH via inhibition of Na-K-adenosine triphosphatase.
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PMID:Effects of vanadate on organic ion accumulation in rat renal cortical slices. 691 88