Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UNIPROT:P20020 (adenosine triphosphatase)
 3,299 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Uvomorulin (E-cadherin), a cell adhesion molecule, and Na+,K(+)-adenosine triphosphatase (ATPase), a marker protein of the basal-lateral cell membrane domains of polarized epithelial cells, were investigated in a group of mouse skin tumors induced by a two-stage chemical carcinogenesis protocol and in cell lines derived from mouse skin papillomas and squamous cell carcinomas (SCC). Although these two markers were present in benign tumors and in nontumorigenic cell lines, the Na+,K(+)-ATPase showed an altered pattern of distribution that included the presence of enzyme not only in the basolateral domain but also on the apical domain of the cell membrane of basal and spinous cells in well-differentiated squamous cell carcinomas (SCC). In higher grade SCC, a loss of Na+,K(+)-ATPase immunoreactivity was simultaneously detected with a marginal or absent expression of uvomorulin. The more differentiated SCC and papillomas expressed less uvomorulin immunoreactivity than normal epidermal cells. Both markers were seen in tumor cell lines that produced well-differentiated SCC after subcutaneous inoculation into nude mice. Neither Na+,K(+)-ATPase nor uvomorulin could be detected in cell lines that produced high grade, poorly differentiated SCC. Northern blots confirmed the absence of uvomorulin mRNA in these highly malignant cell lines. These data indicate that progression from premalignant papilloma to low-grade SCC and subsequently to high-grade SCC is accompanied by loss of epithelial cell polarity as detected by changes in Na+,K(+)-ATPase and by decreased or absent expression of uvomorulin in tumors and cell lines characterized by an advanced malignant phenotype.
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PMID:Alterations in the expression of uvomorulin and Na+,K(+)-adenosine triphosphatase during mouse skin tumor progression. 131 85

The biogenesis of follicles from aggregates of precursor cells is an important morphogenetic process in thyroid embryology. It necessitates the creation of a polarized cell phenotype, assembly of specialized cell-cell junctions, and generation of follicular lumena. In this study we sought to investigate the relationship between cell polarization and lumen formation by studying the cell surface events that occurred when freshly isolated adult porcine thyroid cells reorganized to form follicles in primary culture. Follicular reorganization entailed the initial formation of solid three-dimensional cell aggregates and the subsequent appearance of lumena within aggregates. During the initial stage of cell aggregation, the adhesion molecule, E-cadherin, became expressed at all surfaces involved in cell-cell contact. Aggregation was inhibited by monoclonal antibodies that block cadherin function, indicating directly that E-cadherin is a dominant initial cell-cell adhesion molecule. Cell aggregation was also associated with the recruitment to the cell surface of ZO-1, a tight junction-associated protein, and Na+/K(+)-adenosine triphosphatase. These proteins were initially found throughout regions of cell-cell contact and only subsequently redistributed to their mature locations in tight junctions and the basolateral cell surface, respectively. In contrast, components associated with the apical membrane were first detected within large intracellular vacuoles, which subsequently fused with the cell surface between maturing tight junctions to yield the apical membrane domain and nascent follicular lumena. Follicle formation occurred independently of basal lamina assembly and TSH, although maintenance of follicular architecture required the presence of this hormone. These findings indicate that cultured follicles form in two distinct stages: 1) initial aggregation mediated by E-cadherin and associated with recruitment of components of both tight junctions and the basolateral membrane domain, and 2) subsequent formation of a specialized apical membrane domain by coordinated fusion of intracellular vacuoles at sites of the cell surface where tight junctions are maturing. We propose that follicular morphogenesis may arise as a consequence of epithelial cell polarization within coherent three-dimensional cell aggregates.
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PMID:Cadherin-mediated adhesion and apical membrane assembly define distinct steps during thyroid epithelial polarization and lumen formation. 766 88