Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UNIPROT:P20020 (adenosine triphosphatase)
 3,299 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Methyl isocyanate (MIC) interaction with the rabbit erythrocyte membrane increased the fluidity of the membrane and decreased the osmotic fragility of erythrocytes both in vitro and in vivo in rabbits intoxicated with MIC subcutaneously. MIC inhibited both acetylcholinesterase (AChE) and adenosine triphosphatase (ATPase) activities of erythrocytes dose-dependently in vitro, while in vivo a decreased trend in ATPase activity with unaltered AChE activity was observed. MIC also caused significant decrease in plasma sodium level with corresponding increase in potassium level in rabbits. The observed effects are due to MIC, per se, as the hydrolysis products of MIC, methylamine and N,N'-dimethylurea did not affect the erythrocyte fluidity and enzymes activities both in vitro and in vivo while they increased the osmotic fragility of erythrocytes in vivo in rabbits administered subcutaneously in equimolar concentration to MIC dosage. Inhibition of Na(+)-K(+)-dependent ATPase with altered permeability to cations and also probably water transport of plasma membrane due to MIC interaction are envisaged.
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PMID:Acute toxicity of methyl isocyanate in rabbit: in vitro and in vivo effects on rabbit erythrocyte membrane. 153 91

Mothers who smoke cigarettes during pregnancy give birth to babies with lower birth weights than do nonsmoking mothers. One hypothesis to explain this finding is that nicotine depresses the activity of the placental cholinergic system, which has been linked to the placental transport of amino acids and other substances. The levels and activities of several components of the term placental cholinergic system were determined in smokers and nonsmokers to investigate whether this system is involved in the effect of smoking. There were no statistically significant differences in the levels, synthesis or release of acetylcholine in the tissues from smoking and nonsmoking mothers, nor in the activities of the choline uptake system or the enzymes choline acetyltransferase, cholinesterase or sodium/potassium adenosine triphosphatase. The results do not support the hypothesis that the lower birth weights of babies born to smoking mothers is mediated by an effect of nicotine or other tobacco components on the placental cholinergic system.
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PMID:The effect of cigarette smoking during pregnancy on the cholinergic system in isolated term human placental tissue. 293 60

The mechanism of the anaesthetic effect of toluene on the central nervous system (CNS) was studied by using rat erythrocyte and synaptosome membranes as nerve cell models both in vitro and in vivo. The activities of the membrane-bound integral enzymes acetylcholinesterase (AChE), total adenosine triphosphatase (total ATPase) and magnesium-activated adenosine triphosphatase (Mg2+-ATPase) were determined. A short-term exposure to 2000 p.p.m. of toluene had an inhibitory effect on the enzyme activities studied. The degree of inhibition in erythrocyte membranes in vitro and in vivo, and in synaptosome membranes in vitro were in good correlation. In in vivo conditions, the synaptosome-bound enzymes were, however, significantly more inhibited by toluene, which indicates that membranes in vivo are even more vulnerable to the toxic effects of organic solvents than they are as isolated membranes in vitro. However, our results show that in vitro experiments can be used to predict the toxic nerve cell membrane effects of organic solvents. Toluene caused similar enzyme inhibitions both in neural cell membranes and in erythrocyte membranes. Thus, even peripheral non-excitable cell membranes, like erythrocytes, can be used as nerve cell membrane models in studies on the mechanism of the anaesthesia caused by solvents.
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PMID:The effect of in vitro and in vivo toluene exposure on rat erythrocyte and synaptosome membrane integral enzymes. 296 6

Six rhabdomyosarcomas were assessed by means of a battery of enzyme histochemical methods. The reactions were compared with those of a small number of other tumours belonging to the small-cell tumour category. Four of the rhabdomyosarcomas were positive for myophosphorylase and acetylcholinesterase. Myoblasts were strongly reactive for adenosine triphosphatase at alkaline pH and after acid pre-incubation, whereas the small undifferentiated neoplastic cell of the four alveolar rhabdomyosarcomas showed also discernible cytoplasmic reaction, but only after acid pre-incubation. Other tumour categories revealed positive staining for adenosine triphosphatase with acid pre-incubation but the degree of reaction was minimal by comparison. Other enzyme reactions were variable and, generally, did not distinguish between different tumour categories. It is concluded that enzyme histochemistry has a potential role in the diagnostic evaluation of the small cell tumour and should be included in the growing list of special techniques that may assist the pathologist confronted with this problem.
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PMID:An evaluation of enzyme histochemistry in the diagnosis of childhood rhabdomyosarcoma. 315 41

A cryostat retrieval method and combined adenosine triphosphatase (ATPase) and acetylcholinesterase (AChase) method were used to study the ultrastructure and innervation of histochemically identified skeletal muscle fibers in different pigeon muscles. The Z-line structure and volume percentage sarcotubular system were analyzed from different muscles selected for their composition by fiber type. Histochemically, three main fiber types were investigated: slow tonic fibers with a moderate ATPase activity after preincubation at acid or alkaline pH; fast-twitch fibers that had high activity after alkaline treatment and low activity after acid preincubation; and a type considered to be slow-twitch that had low activity after alkaline, and high after acid preincubation. Both the slow tonic and slow-twitch fibers had multiple, en grappe innervation, while the fast-twitch fibers had robust, single end plates. The Z-line of the fast-twitch and slow-twitch fibers had a regular square lattice pattern, in contrast to the granular, nonlattice structure of the slow tonic Z-line. The volume percentage sarcotubular system of the slow-twitch fibers was intermediate between and significantly different from that of the fast-twitch and slow tonic fibers. These correlative analyses suggest that the avian muscles contain not only the fast-twitch and slow tonic fibers previously known, but also a slow-twitch fiber that appears to be intermediate between the tonic and the mammalian slow-twitch fiber type. Based on the abundance of the sarcotubular system, this fiber type appears to be fast-contracting and -relaxing, in spite of being multiply innervated.
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PMID:Quantitative ultrastructure of histochemically identified avian skeletal muscle fiber types. 361 80

1. Either l-[4,5-(3)H]leucine or [Me-(3)H]choline, or both l-[U-(14)C]leucine and [Me-(3)H]-choline, were injected into the ninth dorsal root ganglion of the frog, and peripheral transport of labelled proteins and/or phospholipids, mostly phosphatidylcholine, was studied by analysis of consecutive segments of the sciatic nerve. 2. At 25 degrees C, approx. 5% of the (3)H-labelled protein was transported at the rate of 152mm/day. The rate was temperature-dependent with the Q(10) value of 2.6. The flow was completely blocked by the local application of colchicine, but was unaffected by cytochalasin D. 3. [Me-(3)H]-Choline was incorporated into phosphatidylcholine at a comparatively slow rate, but was transported in the nerve at a rate equivalent to that for (3)H-labelled proteins. 4. The simultaneous transport of phosphatidylcholine and the protein was further supported in the double-labelling experiments by an identical transport rate of (3)H-labelled phosphatidylcholine and (14)C-labelled proteins, by their identical temperature dependence, by simultaneous blockade with colchicine, and also by the parallel distribution of the two labels in subcellular fractions. Specific radioactivities on a protein basis of both (3)H and (14)C labels were highest in microsomal subfractions enriched with Na(+)-plus-K(+)-stimulated adenosine triphosphatase and acetylcholinesterase. It is suggested that (3)H-labelled phosphatidylcholine and (14)C-labelled proteins transported in the nerve reside in the same structural entity, most probably a membrane component.
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PMID:Rapid transport of phosphatidylcholine occurring simultaneously with protein transport in the frog sciatic nerve. 427 56

Human platelets separated from blood by six different methods have been compared for aggregability, adhesion to glass, adenine nucleotide content and release, and adenosine triphosphatase and cholinesterase activities. Methods of separation of platelets from blood included three differential centrifugation technics, gel filtration and two albumin density gradient methods. Platelets prepared by the different methods aggregated comparably except those separated by albumin density gradient technics which tended to be hyporeactive. Differences in adhesion to glass, adenine nucleotide content and release, and monitored enzyme activities of the various platelet preparations were noted in several cases but were not marked in general. Ultrastructural studies, reported elsewhere, revealed that platelets separated by the method of Mustard or by gel filtration were less altered morphologically than those separated by the other methods. Platelets separated from blood by gel filtration also appeared somewhat superior functionally to platelets separated by other methods.
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PMID:Comparison of certain functions of human platelets separated from blood by various means. 427 71

1. The action of beryllium on the following enzymes has been examined: alkaline phosphatase (Escherichia coli and kidney), acid phosphatase, phosphoprotein phosphatase, apyrase (potato), adenosine triphosphatase (liver nuclei, liver mitochondria, brain microsomes), glucose 6-phosphatase, polysaccharide phosphorylases a and b, phosphoglucomutase, hexokinase, phosphoglyceromutase, ribonuclease, A-esterase (rabbit serum), cholinesterase (horse serum), chymotrypsin. Alkaline phosphatase and phosphoglucomutase are inhibited by 1mum-beryllium sulphate whereas the other enzymes are largely unaffected by 1mm-beryllium sulphate. 2. Possible mechanisms for the inhibition of phosphoglucomutase and alkaline phosphatase are discussed.
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PMID:The inhibition of enzymes by beryllium. 428 87

The foliate papillae of the rabbit, rat and mouse were studied by scanning electron microscopy and histochemistry. The papillae consisted of folds and grooves located on the posterolateral margin of the tongue in front of the circumvallate papillae. The numbers of folds and taste buds varied among the three animals species. Scanning electron microscopy showed that in longitudinal sections the taste buds were oval in shape and their pores were surrounded by microvilli. The reaction product of alkaline phosphatase could only be demonstrated in the superficial epithelium of the rabbit as well as in the mouse foliate papillae, but it also diffused into the taste buds in the rat. The intensity and distribution of the reactions of adenosine triphosphatase, acetylcholinesterase and butyrylcholinesterase were identical to those reported by other investigators in spite of differences in animal species and histochemical techniques employed.
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PMID:Scanning electron microscopic and histochemical studies of foliate papillae in the rabbit, rat and mouse. 621 38

The muscle fibers of the cranial slip of M. pectoralis pars thoracica of an emu (Dromaius novaehollandiae) were studied histochemically for intracellular lipid, succinic dehydrogenase, myofibrillar adenosine triphosphatase, and acetylcholinesterase. It was concluded that the muscle consisted of approximately 28% slow-tonic and 72% fast-twitch glycolytic fibers. The tonic fibers were considered to be characteristic of a postural muscle, and the fast-twitch glycolytic fibers to reflect the inability of the muscle to engage in sustained activity. The general absence of slow-tonic fibers from the pectoralis of other avian species so far studied may be attributed to inadequate sampling of the deeper regions of the muscle.
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PMID:Some histochemical properties of the fiber types in the pectoralis muscle of an emu (Dromaius novaehollandiae). 623 56


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