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Query: UNIPROT:P17931 (
galectin-3
)
2,860
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Although mounting evidence indicates the involvement of
galectin-3
in cancer progression and metastasis, the underlying molecular mechanisms remain largely unknown. In this study, we investigated the effect and possible mechanism of
galectin-3
on the migration and invasion of B16F10, a metastatic melanoma cell line, in which
galectin-3
and matrix metalloproteinase- 1 (MMP-1) were both found to be highly expressed. Knockdown of
galectin-3
with specific siRNA reduced migration and invasion, which was associated with reduced expression of MMP-1. To further investigate the underlying mechanism, we examined the effect of
galectin-3
knockdown on the activity of AP-1, a
transcriptional factor
regulating MMP-1 expression. We found that
galectin-3
directly interacted with AP-1 and facilitated the binding of this complex to the MMP-1 promoter that drives MMP-1 transcription. Moreover, silencing of
galectin-3
inhibited binding of fra-1 and c-Jun to promoter sites of MMP-1 gene. Consistent with these in vitro findings, our in vivo study demonstrated that
galectin-3
shRNA treatment significantly reduced the total number of mouse lung metastatic nodules. Taken together, galectin- 3 facilitates cell migration and invasion in melanoma in vitro and can induce metastasis in vivo, in part through, regulating the transcription activity of AP-1 and thereby up-regulating MMP-1 expression.
...
PMID:Galectin-3 increases the motility of mouse melanoma cells by regulating matrix metalloproteinase-1 expression. 2243 31
Galectin-3
is a carbohydrate-binding protein and regulates diverse functions, including cell proliferation and differentiation, mRNA splicing, apoptosis induction, immune surveillance and inflammation, cell adhesion, angiogenesis, and cancer-cell metastasis.
Galectin-3
is also recommended as a diagnostic or prognostic biomarker of various diseases, including heart disease, kidney disease, and cancer.
Galectin-3
exists as a cytosol, is secreted in extracellular spaces on cells, and is also detected in nuclei. It has been found that
galectin-3
has different functions in cellular localization: (i) Extracellular
galectin-3
mediates cell attachment and detachment. (ii) cytosolic
galectin-3
regulates cell survival by blocking the intrinsic apoptotic pathway, and (iii) nuclear
galectin-3
supports the ability of the
transcriptional factor
for target gene expression. In this review, we focused on the role of
galectin-3
on translocation from cytosol to nucleus, because it happens in a way independent of carbohydrate recognition and accelerates cancer progression. We also suggested here that intracellular galecin-3 could be a potent therapeutic target in cancer therapy. [BMB Reports 2020; 53(4): 173-180].
...
PMID:Non-classical role of Galectin-3 in cancer progression: translocation to nucleus by carbohydrate-recognition independent manner. 3217 30
