Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UNIPROT:P17174 (aspartate aminotransferase)
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To purify cytoplasmic and mitochondrial isoenzymes of aspartate aminotransferase (EC 2.6.1.1) from human liver. We used heat treatment, ammonium sulfate precipitation, anion- and cation-exchange chromatography, affinity chromatography, and isoelectric focusing. Final preparations of the isoenzymes were homogeneous, with specific activities of 198 and 208 kU/g for the cytoplasmic and the mitochondrial enzymes, respectively. The mitochondrial isoenzyme focused as a single band with a pl value of 9.60, whereas the cytoplasmic isoenzyme had subforms with pl values of 5.22, 5.42, and 5.62 at 4 degrees C. In Tris . HCl buffer, both isoenzymes have an activity maximum at pH 7.8. In [bis(2-hydroxyethyl)amino]tris(hydroxymethyl)methane (Bistris) buffer, however, the mitochondrial isoenzyme also showed an optimum pH of 6.7.
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PMID:Isolation and purification of aspartate aminotransferase isoenzymes from human liver by chromatography and isoelectric focusing. 746 Feb 73