Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: UNIPROT:P17174 (aspartate aminotransferase)
 14,872 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Indirubin-3'-oxime is an indirubin analogue that shows favorable inhibitory activity targeting glycogen synthase kinase 3beta (GSK-3beta). In this study, we evaluated if acute treatment with indirubin-3'-oxime (Ind) prevents hepatic ischemia/reperfusion (I/R) damage. Wistar rats were subjected to 150 min of 70% warm ischemia and 16 h of reperfusion. In the treated group 1 microM indirubin-3'-oxime was administered in the hepatic artery 30 min before ischemia. Acute treatment with Ind decreased serum alanine aminotransferase (ALT), aspartate aminotransferase (AST) and lactate dehydrogenase (LDH) levels, comparatively to I/R livers. Bax translocation to the mitochondria and cytochrome c release were higher in I/R livers. Ind treatment significantly attenuated Bax translocation and preserved mitochondrial cytochrome c content. Ind also protected mitochondria from calcium-induced mitochondrial permeability transition (MPT), as well as the decrease in state 3 mitochondrial respiration, the delay in the repolarization after a phosphorylative cycle and the decrease in ATP content caused by I/R. By addressing GSK-3beta activity and phosphorylated GSK-3beta at Ser(9) content in liver homogenates and isolated mitochondria, data suggests that inhibition of GSK-3beta by indirubin-3'-oxime prevents the increase in mitochondrial phosphorylated GSK-3beta at Ser(9) induced by I/R, thus correlating with MPT inhibition and preservation of cytochrome c content. Pre-treatment with indirubin-3'-oxime in conditions of hepatic I/R, protects the liver by maintaining mitochondrial function and hepatic energetic balance.
 ...
PMID:Indirubin-3'-oxime prevents hepatic I/R damage by inhibiting GSK-3beta and mitochondrial permeability transition. 2043 52

Recently, there has been as enhanced interest on global level to recognize the potent antioxidant compounds which are pharmacologically active with less or no side effects. Thus, the current investigation was intended to scrutinize the protective effect of indirubin on the cardiac marker, such as, enzymes, LDH isoenzyme, cardiac troponin-T (cTnT), antioxidant enzymes marker and lipid peroxidation (LPO) in response of isoproterenol (ISO)-induced myocardial infarction (MI) in Wistar rats. The experimental animals were categorized into following groups: Group I received saline; Group II received Indirubin (10 mg/kg); Group III received ISO (100 mg/kg) and Group IV received ISO + indirubin (10 mg/kg) for continuous 10 days. The ISO induced MI injury was confirmed via enhanced level of enzymes markers viz., creatine kinase-MB, creatine kinase, lactate dehydrogensae, troponin-T, alanine transaminase (ALT) and aspartate transaminase (AST) in the rats serum. The enhanced expression of LDH (1 and 2) isoenzyme bands were also observed in the ISO induced MI rats. We have also estimated the level of LPO in the heart and plasma, which was found to be significantly (P<0.05) improved. Moreover, the marker of enzymatic antioxidant enzymes viz., glutathione reductase (GRx), catalase (CAT), glutathione-S-transferase (GST), glutathione peroxidase (GPx) and superoxide dismutase (SOD) in the heart, and the level of non-enzymatic antioxidant marker viz., vitamin (C, E) in heart and serum were found to be considerably (P<0.05) reduced in the ISO induced MI in Wistar rats. Whereas, the ISO control Wistar rats showed significant (P<0.05) increase in the uric acid level in the plasma. The Indirubin treated rats confirmed the significant protective effect via modulation of all biological and antioxidant parameters tested. The result of the investigation was further found in agreement of the histopathological studies of the indirubin treated rats which clearly showed recovery from the myocardial infarction. Thus, on the basis of that, it has been suggested that indirubin showed protection of myocardial tissues against the ISO persuaded oxidative stress.
 ...
PMID:Cardioprotective effect of indirubin in experimentally induced myocardial infarction in wistar rats. 3196 61