Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UNIPROT:P17174 (aspartate aminotransferase)
 14,872 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Normal values for 13 chemical constituents of plasma were estimated from results for 837 presumably healthy children. Ninety microliters of specimen was analyzed for lactate dehydrogenase, aspartate aminotransferase, alkaline phosphatase, inorganic phosphorus, total calcium, total cholesterol, total proteins, albumin, uric acid, urea nitrogen, alanine aminotransferase, total bilirubin, and glucose. We used two Abbott ABA-100 Bichromatic Analyzers interfaced directly to the ABA Data Management System. For each test age- and sex-related variations were assessed and normal values were estimated for six different age groups.
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PMID:Microchemical analysis for 13 constituents of plasma from healthy children. 43 35

Measurements of alanine aminotransferase and aspartate aminotransferase with the SMAC were evaluated for correlation with the ABA-100, precision, linearity, and carryover. We assayed 200 specimens with normal and abnormal aminotransferase activities with both the SMAC (y) and the ABA-100 (X). Linear regression analysis of the data yielded the following: alanine aminotransferase (r = 0.9732, y = 0.96x + 3.8); and aspartate aminotransferase (r = 0.9892, y = 0.90x + 2.1). Both aminotransferases demonstrated acceptable intra- and inter-assay variations with the SMAC and ABA-100. With the SMAC the upper limit of linearity for alanine aminotransferase was 350 U/L; that for asparate aminotransferase was 450 U/L. Carryover studies for SMAC indicate that specimens immediately following specimens with alanine aminotransferase activities greater 400 U/L and (or) aspartate aminotransferase activities greater than 500 U/L should be re-analyzed.
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PMID:Alanine aminotransferase and aspartate aminotransferase measurements with two automated analyzers, SMAC and the ABA-100, compared. 43 50

The overall performances of several enzyme reagent kits for alkaline phosphatase, creatine kinase, lactic dehydrogenase, and aspartate aminotransferase were evaluated using an ABA-100 Bichromatic Analyzer. Interassay precision using this instrument with commercial reagents compared well with published data for similar analyses performed at university hospitals and referral laboratories. Significantly poorer precision with lower limits of linearity was observed when reagents recommended for use at 30 C were used at 37 C. Significant differences in measured levels of creatine kinase, lactic dehydrogenase, and aspartate aminotransferase due to different lots of expendable cuvettes were found for elevated levels of these enzymes. All kit reagents met manufacturers' claims for stability; however, different absolute levels of lactic dehydrogenase were observed with one kit reagent on successive days. Slight hemolysis affected creatine kinase levels measured with some reagent kits significantly more than others.
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PMID:Evaluation of commercial enzyme reagent kits by use of a semiautomated chemistry analyzer. 47 90

This report summarizes a one year evaluation of Abbott's ABA 100, with respect to mechanical parts (syringe plates, precision and linearity of photometry, band width of several filters, multicuvet precision, temperature control) and the reliability of several methods (endpoint procedures: determination of the glucose concentration with hexokinase- and the glucose dehydrogenase method, and of the protein concentration; enzyme activities: alanine and aspartate aminotransferase, creatine kinase, alkaline phosphatase). The critical batch size was estimated as an indicator of economy (about 40 samples per day for the glucose concentration). Various aspects of the instrument are discussed with respect to its use in clinical chemistry.
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PMID:Evaluation of the Abbott Bichromatic Analyzer 100 (A proposal for an evaluation scheme). 95 29

Forty-three kits for clinical chemical analysis of GOT (aspartate aminotransferase) were evaluated. The reference method was the kinetic method performed on the Abott ABA-100 Bichromatic Analyzer. Specific data are tabulated for each kit and include ease and speed of testing and performance data. Most kits failed to provide completely adequate labelling information. Reproducibility CV for the kits varied widely (4.20 to 21.26 for the normal pool and 1.57 to 20.51 for the high pool). Many kits gave significant numbers of false positive and false negative results on patient samples. There is a need for manufacturers to either derive their own normal ranges or to establish exact equivalency values with the method used to establish the normal values. No difference was demonstrated between performance by two skilled technologists. However, we did detect lot-to-lot variation and differences in performance between narrow bandpass and wide bandpass spectrophotometers.
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PMID:Performance of "kits" used for clinical chemical analysis of GOT (aspartate aminotransferase). 126 Oct 5

The performance characteristics of the Scandinavian Committee on Enzymes (SCE) methods for the assay of aspartate aminotransferase (AST) and alanine aminotransferase (ALT) were determined using six automated enzyme analysers. The reagent formulation did not include pyridoxal phosphate (PLP). An optimal operating mode was defined for each instrument and precision was assessed in greater detail on four instruments. A points rating system was devised to place the instruments in the following order of proficiency: IL Multistat III, LKB-8600, Gilford 3500, ABA 100. In contrast to AST, the ALT activity of patient samples was unstable at -20 degrees C over periods as short as seven days. The performance characteristics of the IFCC methods for assay of AST and ALT activities were determined by using three automated enzyme analyzers in order to assess the effect of PLP upon precision and activity of four quality control sera, and to compare the SCE and IFCC methods. Precision of AST assays did not alter on omission of PLP from the IFCC formulation, while that of ALT assays showed slight deterioration. The decrease in activity on omitting PLP was variable with each instrument. A points-rating system was devised to place the methods in the following order of precision: AST: IFCC (-PLP) 118, IFCC 109, SCE 61; ALT: IFCC 125, IFCC (-PLP) 97, SCE 66. The IFCC methods offer better precision, and the overall change on omitting PLP is minimal.
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PMID:Evaluation of commercially formulated aspartate aminotransferase and alanine aminotransferase activity determinations by the Scandinavian Committee on Enzymes and IFCC methods as modified for use with automated enzyme analysers. 323 38

Serum aspartate aminotransferase (AST) activity was measured by the methods recommended by the Scandinavian Committee on Enzymes (SCE) and by the International Federation of Clinical Chemistry (IFCC) with pyridoxal phosphate (PLP) and without (-PLP) in one laboratory at 37 degrees C with the Abbott ABA-100 and in another at 30 degrees C with the IL Multistat III. Reference ranges were determined on 195 healthy hospital staff. Sera from 102 patients with suspected hepatobiliary disease (HBD) and 104 with suspected myocardial infarction (MI) were assayed at both laboratories by all three methods. Based on the above reference ranges, all assays with each method at both hospitals were abnormal in 59 of 67 cases with HBD and 53 of 55 with MI. In aggregate, all three methods yielded comparable rates of misclassification (20-23). The SCE method gave highest false negatives (18) and lowest false positives (5); the IFCC method gave lowest false negatives (1) and highest false positives (20); intermediate values of 8 false positives and 12 false negatives were given by the IFCC (-PLP) method. Using receiver operating characteristic (ROC) curves, the SCE method was clearly superior at 30 degrees C, and the IFCC (-PLP) method was marginally superior at 37 degrees C. However, when the decision threshold corresponded with a 2.5% false positive rate in the non-HBD, non-MI patients, the SCE method gave the lowest false negatives at both temperatures and, on the basis of the present data, must be considered to be the method of choice for AST activity determinations.
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PMID:The diagnostic accuracy of three recommended methods for serum aspartate aminotransferase assays in patients suspected of myocardial infarction and hepatobiliary diseases. 323 44