Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: UNIPROT:P17174 (
aspartate aminotransferase
)
14,872
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Introduction of a C
4
photosynthetic pathway into C
3
rice (
Oryza sativa
) requires installation of a biochemical pump that concentrates CO
2
at the site of carboxylation in modified bundle sheath cells. To investigate the feasibility of this, we generated a quadruple line that simultaneously accumulates four of the core C
4
photosynthetic enzymes from the NADP-malic enzyme subtype, phospho
enol
pyruvate carboxylase (
Zm
PEPC), NADP-malate dehydrogenase (
Zm
NADP-MDH), NADP-malic enzyme (
Zm
NADP-ME), and pyruvate phosphate dikinase (
Zm
PPDK). This led to enhanced enzyme activity and mild phenotypic perturbations but was largely neutral in its effects on photosynthetic rate. Measurements of the flux of
13
CO
2
through photosynthetic metabolism revealed a significant increase in the incorporation of
13
C into malate, consistent with increased fixation of
13
CO
2
via PEP carboxylase in lines expressing the maize PEPC enzyme. However, there was no significant differences in labeling of 3-phosphoglycerate (3PGA) indicating that there was no carbon flux through NADP-ME into the Calvin-Benson cycle. There was also no significant difference in labeling of phospho
enol
pyruvate (PEP) indicating that there was no carbon flux through PPDK. Crossing the quadruple line with a line with reduced glycine decarboxylase
H-protein
(
Os
GDCH) abundance led to a photosynthetic phenotype characteristic of the reduced
Os
GDCH line and higher labeling of malate, aspartate and citrate than in the quintuple line. There was evidence of
13
C labeling of aspartate indicating
13
CO
2
fixation into oxaloacetate by PEPC and conversion to aspartate by the endogenous
aspartate aminotransferase
activity. While Kranz anatomy or other anatomical modifications have not yet been installed in these plants to enable a fully functional C
4
cycle, these results demonstrate for the first-time a partial flux through the carboxylation phase of NADP-ME C
4
metabolism in transgenic rice containing two of the key metabolic steps in the C
4
pathway.
...
PMID:A Partial C
4
Photosynthetic Biochemical Pathway in Rice. 3317 34
