Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: UNIPROT:P15088 (mast cell)
 14,925 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Treatment with carboxypeptidase A of ribulose bisphosphate carboxylase/oxygenase (rubisco) from spinach and Chlamydomonas, but not tobacco, reduced activity by 60-70%. Further studies with the spinach enzyme indicated that only one amino acid from each of the large (valine) and small (tyrosine) subunits was removed and the loss of activity was correlated with modification of the large subunit. The modified enzyme also had a two-fold greater Km for RuBP but CO2/O2 specificity was only 5% lower and may not be significantly different. The relative rates of release of valine and tyrosine also depended on the presence or absence of RuBP or CO2 plus Mg during treatment. The results indicate that the C-terminal amino acid in the large subunit of spinach, which is not located near the active site region, plays a previously unrecognized role in determining the catalytic activity of the enzyme.
 ...
PMID:Partial reduction in ribulose 1,5-bisphosphate carboxylase/oxygenase activity by carboxypeptidase A. 227 51

Trypsin-catalysed cleavage of purified ribulose 1,5-bisphosphate carboxylase/oxygenase (Rubisco) and the resultant irreversible loss of carboxylase activity were prevented by prior incubation with the naturally occurring nocturnal Rubisco inhibitor 2'-carboxy-D-arabitinol 1-phosphate (CA1P), as well as with ribulose 1,5-bisphosphate (RuBP), Mg2+ and CO2. CA1P also protected Rubisco from loss of activity caused by carboxypeptidase A. When similar experiments were carried out using soluble chloroplast proteases, CA1P was again able to protect Rubisco against proteolytic degradation and the consequent irreversible loss of catalytic activity. Thus, CA1P prevents the proteolytic breakdown of Rubisco by endogenous and exogenous proteases. In this way, CA1P may affect the amounts of Rubisco protein available for photosynthetic CO2 assimilation. Rubisco turnover (in the presence of RuBP, Mg2+ and CO2) may confer similar protection against proteases in the light.
 ...
PMID:2'-carboxy-D-arabitinol 1-phosphate protects ribulose 1, 5-bisphosphate carboxylase/oxygenase against proteolytic breakdown. 1058 77