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Query: UNIPROT:P15088 (mast cell)
 14,925 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Clinical evidence for the phagocytic capability of neoplastic feline mast cells was provided by recognition of endocytosed erythrocytes in seven of 12 cytological smears of mast cell neoplasms, particularly in those cells collected from splenic tumors. The capability of these neoplastic mast cells to endocytose particulate substances was also studied in vitro. Evidence is presented that under cultural conditions, feline neoplastic mast cells are capable of endocytosing a variety of substances including polystyrene latex microspheres, zymosan particles, horse spleen ferritin, salmon sperm nuclei, horseradish peroxidase, and carbon particles.
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PMID:Endocytosis of erythrocytes in vivo and particulate substances in vitro by feline neoplastic mast cells. 313 17

Sixty-five canine skin neoplasms studied using immunocytochemistry, included 22 histiocytomas, 18 amelanotic melanomas, 14 cutaneous lymphosarcomas, six mast cell tumors, and five transmissible venereal tumors. Formalin-fixed, paraffin-embedded sections were stained using the avidin-biotin-peroxidase complex (ABC) immunoperoxidase technique for reactivity with S-100 protein, kappa and lambda immunoglobulin light chains, alpha-1-antitrypsin, alpha-1-antichymotrypsin, leukocyte common antigen (LCA), neuron-specific enolase, keratin, cytokeratin, muramidase, and vimentin. Detection of S-100, kappa and lambda light chains, neuron-specific enolase, and vimentin were most useful for screening these neoplasms. None of the markers examined was consistent in staining histiocytomas. While reactivity of S-100 (ten cases) and neuron-specific enolase (ten cases) was detected in some amelanotic melanomas, lambda light chain immunoglobulin (eight cases) was relatively consistent in cutaneous lymphomas. Mast cell neoplasms reacted with avidin and, therefore, were positive, even on negative control sections. Vimentin reacted strongly on all amelanotic melanomas and transmissible venereal tumors examined. These antibodies are helpful adjuncts in the differential diagnosis of canine skin tumors.
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PMID:Diagnostic immunohistochemistry of canine round cell tumors. 313 15

The response to daily topical applications of arachidonic acid (0.25-4 mg/ear/day) to the ears of outbred CD-1 mice was monitored. The first application produced erythema, extravasation of plasma proteins resulting in an increase in ear weight, and some neutrophil accumulation (detected histologically and quantified by myeloperoxidase content). The second application produced minimal edema but did cause erythema and a greater accumulation of neutrophils. Subsequent daily application caused erythema, neutrophil accumulation, and an increase in ear weight predominantly due to cell proliferation (epidermis and connective tissue). Daily applications of other unsaturated fatty acids did not match the response induced by arachidonic acid. Mast cell deficient mice (W/Wv) exhibited a smaller edema response to the first dose of arachidonic acid compared to either their wild-type controls or CD-1 mice. In addition, W/Wv mice exhibited a smaller ear weight increase and myeloperoxidase accumulation following eight daily doses of arachidonic acid. However, epidermal proliferation was similar in all the strains of mice tested. These data suggest that the edema caused by the first topical application of arachidonic acid is partly mast cell mediated. Mast cells also appear to be involved in the neutrophil infiltration induced by multiple topical applications, but not in the epidermal proliferation.
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PMID:Multiple topical applications of arachidonic acid to mouse ears induce inflammatory and proliferative changes. 313 72

The morphological characteristics and lectin-binding properties of mast cell granules from four human neurofibromata are described. Ultrastructural examination of the granules revealed that some contained dense cores, others had membranous configurations and some forms were intermediate between the two. A round electron-lucent area was present in some granules. After treatment with biotinylated lectins (10 micrograms ml-1) followed by an avidin-peroxidase revealing system (5 micrograms ml-1 in 0.125 M Tris-buffered saline with 0.347 M NaCl, pH 7.6), mast cell granules strongly bound Concanavalin A, garden pea, lentil, wheatgerm, erythro- and leuco-kidney bean lectins. This indicated the presence of abundant N-linked complex-type saccharide sequences. Soybean and peanut lectins showed only weak binding, while the presence of sparse alpha-L-fucosyl terminals was indicated by the weak binding of winged pea lectin. The staining intensity of wheatgerm lectin was considerably reduced when incubated in the presence of its specific competing sugar tri-N-acetylchitotriose. Despite a wide variety of morphological differences between granules, all showed similar staining patterns and all granules within a single cell shared the same binding characteristics.
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PMID:An ultrastructural study of the morphology and lectin-binding properties of human mast cell granules. 319 20

Cutaneous mast cell degranulation in rats results in tissue inflammation, and this species has therefore provided a useful model to study the pathogenesis of late phase reactions (LPRs). The mast cell dependency of LPRs has been confirmed by the demonstration that isolated rat mast cell granules (MCGs), when injected intradermally into rat skin, induce patterns of tissue inflammation similar to those seen after skin testing with anti-IgE antibody. Rat LPRs are neutrophil dependent, and, further, MCG-derived inflammatory factors can chemically attract rat neutrophils in vitro. To further study the relationships among MCGs, tissue inflammation, and neutrophil function, luminol-dependent chemiluminescence (CL) responses of rat peritoneal-elicited neutrophils in response to opsonized zymosan and phorbol myristate acetate (PMA) in the presence and absence of MCGs were analyzed. When MCGs (1.0, 10, and 100 micrograms/ml) alone were added to neutrophil suspensions, a rapid concentration-dependent increase in baseline CL responses was observed; these increases (maximum of sixfold) were modest, varied with cell concentrations, and followed different time courses compared with those seen after addition of preopsonized zymosan (0.5 mg/ml) (50-fold increases that peaked in 4 to 8 minutes). However, if neutrophils were preincubated (15 minutes) in the presence of MCGs, the CL response to opsonized zymosan (1.25 mg/ml) was significantly and synergistically enhanced compared with the response seen with MCGs alone. Similar but less pronounced effects were also noted after cell activation with PMA (2.5 and 25 ng/ml). To determine which component of the MCG was responsible for this enhancing activity, additional experiments were performed. Enhancement was still observed, albeit less intense, if MCGs were prepared membrane free and washed free of readily dissociable mediators such as histamine. Histamine (10(-6) and 10(-5) mol/L) had no enhancing effect nor did preparations of MCG membranes. MCG solubilization (3 mol/L NH4HCO3) revealed that the enhancing activity resided completely in the high molecular weight (greater than 10,000 daltons) fraction. Heat treatment of the granules and sodium azide preincubation completely abolished the enhancing effect. Exogenous horseradish peroxidase, at peroxidase activity levels contained within the MCGs (1 x 10(-4) to 10(-2) U/ml), reproduced the enhancing effect. After opsonized zymosan activation, neutrophils generated less H2O2 and superoxide anion in the presence of MCGs.(ABSTRACT TRUNCATED AT 400 WORDS)
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PMID:Mast cell granule enhancement of neutrophil chemiluminescence responses. 334 48

The purpose of this study was to determine if human mast cell granules contain non-repeating oligosaccharide sequences. The binding of lectins to human mast cell granules was studied using a panel of 11 lectins variously selective for both N- and O-linked oligosaccharide sequences. The tissues were principally derived from cutaneous neurofibromata and benign and malignant breast diseases, that is, readily available human material with a known high content of mast cells. Lectin-binding sites in the formalin-fixed paraffin-embedded or resin-embedded material was visualized by means of biotinylated lectins and an avidin-peroxidase technique for light microscopy. The results indicate that human mast cell granules contain abundant N-linked sequences, but few or no O-linked residues. These sequences appear to be mostly in the form of non-bisected highly branched or smaller biantennate sequences, although variable positive binding with erythrophytohaemagglutinin was observed, indicating some degree of bisection.
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PMID:Lectin histochemistry of the mast cell: a light microscopical study. 341 Jul 38

An autopsy case of systemic mastocytosis without cutaneous involvement in a 76-year-old woman was described. The patient presented with general malaise, chest and epigastric discomfort, flushing of the face and progressive hepatosplenomegaly, and she terminated in hemorrhagic complications of DIC within 2 months. There was neither rash nor urticaria pigmentosa recognizable in the entire course. The diagnosis was made by the histologic identification of abnormal aggregates of mast cells in a bone marrow aspirate. These mast cell granules were chloroacetate esterase-positive, peroxidase-negative, and electronmicroscopically they were composed of fine granular materials containing variable numbers of lamellar structures. At autopsy, diffuse infiltration of the mast cells was found in the liver, spleen, bone marrow, lymph nodes, lungs, kidneys, stomach, and adrenal glands.
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PMID:Systemic mastocytosis without cutaneous involvement. 355 89

The role of mast cells in induction of uterine eosinophilia was investigated by using genetically mast cell-deficient (WB X C57BL/6)F1-W/Wv (hereafter called WBB6F1-W/Wv) mice. The injection of estradiol-17 beta (0.16 micrograms/g body weight) increased the peroxidase activity and eosinophil number in the uteri of castrated WBB6F1-W/Wv and WBB6F1-+/+ mice. Since no significant differences were detectable between these two type of mice, mast cells did not seem to be essential for the estrogen-induced uterine eosinophilia, at least in mice.
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PMID:Estrogen-induced increase in eosinophil number and peroxidase activity in uterus of genetically mast cell-deficient W/Wv mice. 408 34

The vascular permeability-increasing action of rabbit PMNL lysosomes has been studied in skin and cremaster muscle of the rat. Both an extract of frozen-thawed granules and a cathepsin-free cationic protein fraction of the granules (which had previously been demonstrated to cause leukocyte adhesion and emigration in vivo) induce increased vascular permeability in skin and muscle which resembles that produced by histamine or histamine-liberators with respect to the timing of the response and the predominant type of microvessel affected. Extracts of frozen-thawed lysosomes and the inflammatory lysosomal cationic protein both cause disruption of rat mesenteric mast cells in vitro, whereas a granule-free cytoplasmic fraction of PMN leukocytes and a non-inflammatory cationic protein fraction of the granules do not do so under identical test conditions. The mastocytolytic action of lysosomal materials in vitro is not inhibited in the presence of 10 kallikrein-inhibiting units of trasylol per ml. The mast cell rupturing fraction of PMNL granules (cationic protein) possesses no detectable peroxidase activity or acid-mucopolysaccharase activity. When compared with compound 48/80 on the basis of estimated molecular weight, the lysosomal cationic protein appears to be at least as active as the latter compound with respect to in vitro mastocytolytic potency. Chronic pretreatment of rats with an agent known to reduce tissue mast cell numbers causes marked suppression of the vascular permeability change normally induced in skin and muscle by lysosomal extracts and cationic protein. Similar results are obtained if lysosomal materials are tested in rats pretreated with an antihistaminic. These observations are discussed with respect to the mode of action of PMNL lysosomes in the early and late phases of local tissue-injury reactions.
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PMID:Mediators of inflammation in leukocyte lysosomes. II. Mechanism of action of lysosomal cationic protein upon vascular permeability in the rat. 437 82

As demonstrated by labeling with peroxidase, avidin was found to bind selectively and distinctly to mast cell granules. Inhibition studies suggested that avidin is bound by heparin. Based on this new mast cell staining procedure, mast cell distribution in the inflamed synovium of rheumatoid arthritis (RA) and osteoarthritis (OA) has been investigated. In the subsynovial layer, a significant decrease in mast cell numbers was observed in RA-synovium when compared with OA-synovium. This decrease correlated with the presence of lining cell ulcers and granulation tissue and can be interpreted as the result of mast cell degranuation induced by complement-mediated or immune complex-triggered mechanisms.
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PMID:Analysis of mast cells in rheumatoid arthritis and osteoarthritis by an avidin-peroxidase staining. 608 57


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