Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: UNIPROT:P15088 (mast cell)
 14,925 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Metrizamide-gradient purified rat peritoneal mast cells were allowed to adhere to schistosomula of S. mansoni that had been preincubated in either minimum essential medium with 5% fetal calf serum (MEM/FCS), heat-inactivated serum from rats 8 weeks after cercarial infection (IRS) fresh normal rat serum as a source of complement (NRS), or IRS and NRS. Adherence was evaluated in sections 0.3 micrometers thick. The preincubation conditions favoring cell adherence were IRS + NRS greater than NRS greater than IRS greater than MEM/FCS. Greater adherence was seen at 60 min than at 10 min. Discharge of mast cell granulates was seen infrequently, was not greater in adherent than nonadherent cells, was not increased by any preincubation condition, and did not occur against the parasite's surface, as would be expected if antigen, antibody, and surface receptors were aggregated there. Electron microscopic examination showed that attachment to the parasite occurred through electron-dense material which was usually fibrillar in appearance. Membrane fusion, such as is seen between human neutrophils and schistosomula, did not occur.
 ...
PMID:Light and electron microscopic appearance of rat peritoneal mast cells adhering to schistosomula of Schistosoma mansoni by means of complement or antibody. 732 50

The postformalin ammoniacal silver (AS) and the ethanolic phosphotungstic acid (EPTA) methods were used to localize basic protein at the ultrastructural level in cytoplasmic granules of rat eosinophils and mast cells isolated using a Metrizamide gradient. Intense reaction was seen in the granules of EPTA-treated eosinophils. Following incubation of the cells for 2 hr in EPTA alone, the matrix was stained. After longer incubation (10 hr), however, both the matrix and core were stained. Cytoplasmic granules of the mast cell show a slight or negative reaction with EPTA. With the AS technique, a large number of silver particles were seen in the nucleus of both eosinophils and mast cells. The mast cell cytoplasmic granules showed intense reaction, while those from eosinophils showed no clear reaction. Acetylation of the cells under conditions sufficient to block most free amino groups prio to EPTA or AS treatment greatly reduced (EPTA) or abolished (AS) the reaction. The results indicate 1) that eosinophil granules contain basic proteins both in the matrix and the core, 2) that the mast cell granules contain a basic protein (probably the alpha-chymotrypsin-like enzyme), which reacts strongly with AS, and 3) that the AS and EPTA methods have different specificities.
 ...
PMID:Ultrastructural localization of basic proteins in cytoplasmic granules of rat eosinophils and mast cells. 735 18