Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UNIPROT:P15088 (mast cell)
 14,925 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Human adenoids and tonsils were disintegrated mechanically and the cells dispersed by passage through a stainless-steel screen in EDTA-containing buffer. Collagenase digestion did not increase the yield of adenoidal cells. The mast cell content of the cell suspensions was in the range of 1-10 mast cells/10(4) cells with an estimated mean of 1-2 mast cells/10(4) cells, a value considerably below previous reports on adenoidal cell suspensions. The mast cell content was determined by staining with toluidine blue at low pH (to prevent interference by phagocytes). The mast cell count as assessed by alcian blue staining and by fluorescence microscopy after FITC-anti-human IgE binding was similar. Various attempts to enrich the cell suspension (i.e. by differential centrifugation, by gradient centrifugation on Ficoll or Ficoll-Hypaque and by velocity sedimentation at unit gravity) all gave negative results.
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PMID:On the isolation of mast cells from human adenoids and tonsils. 360 1

The events associated with antigen-induced, IgE-mediated degranulation of human basophils from allergic donors were studied ultrastructurally. Partially purified cells were examined prior to addition of antigen and after incubation with antigen or control buffer for 15 seconds to 60 minutes. Reactions were stopped instantaneously by adding fixative directly to the cell suspension. After fixation the cells were exposed to cationized ferritin as a sensitive probe for demonstrating possible continuities between the cytoplasmic granules and the cell surface. Ficoll-Hypaque-isolated basophils were of three types: Type I, cells containing basophil granules with a full complement of particles; type II, cells containing some full granules but also variable numbers of cytoplasmic vacuolar structures having the size and shape of basophilic granules but having reduced or no particle content (partially fixed or empty granules); and type III, basophils containing only empty granules. Following exposure to specific antigen, basophils of all three types underwent degranulation characterized by the fusion of the membranes bounding single basophilic granules with the plasma membrane and leading to extrusion of content. Cells in the process of degranulation (type IV and V basophils) were characterized by communications between individual granules and the cell exterior. Identification of such communications was facilitated by cationized ferritin which entered granules having open communications with the cell surface. Without this marker, the number of such communications would have been seriously underestimated, either because they were extremely narrow, tortuous, or outside the plane of section. The majority of individual basophilic granules fused singly and separately with the plasma membrane, in contrast to guinea pig basophil and rat mast cell degranulation where intercommunicating clusters of granules fused with the plasma membrane at a single point. The particle and membrane contents of extruded granules frequently remained adherent to the surface of type IV and V basophils and were not immediately solubilized. Morphologic evidence of degranulation progressed with time of exposure to antigen, exhibiting kinetics that paralleled histamine release. Cells in control incubations, and rare basophils that had been exposed to antigen E, failed to degranulate. Fully degranulated (type VI) basophils were viable cells that had a markedly irregular surface and were devoid of basophilic granules but retained the minor population of small perinuclear granules.
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PMID:Antigen-induced IgE-mediated degranulation of human basophils. 615 54